HPAEC-PAD法によるピアルロン酸オリゴ糖の分離と定量
書誌事項
- タイトル別名
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- Separation and Quantitative Analysis of Hyaluronan Oligosaccharides by HPAEC-PAD.
- HPAEC-PAD法によるヒアルロン酸オリゴ糖の分離と定量
- HPAEC-PADホウ ニ ヨル ヒアルロンサン オリゴトウ ノ ブンリ ト テイリョウ
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抄録
Hyaluronan oligosaccharides were analyzed using high-performance anion-exchange chromatogra-phy with pulsed amperometric detection (HPAEC-PAD) . The procedure invalves the separation of hyaluronan oligosaccharides on an anion-exchange column, CarboPac PA-1, using alkaline eluants. A gradient is made using these two solutions; solution A (100 mM sodium hydroxide) and solution B (100 mM sodium hydroxide/1.5 M sodium acetate), eluted at 1 mL/min. The unit resolution of hyaluronan oligomers could be achieved up to at least 20 disaccharide units through HPAEC-PAD. Furthermore, integrated pulsed amperometry detected only -40 pmol of hyaluronan oligosaccharide. This analysis system was easily used for the separation and quantitative analysis of hyaluronan oligosaccharides, and applied to the determination of the time course of hyaluronidase digestion of hyaluronan.
収録刊行物
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- Journal of Applied Glycoscience
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Journal of Applied Glycoscience 46 (3), 299-302, 1999
日本応用糖質科学会
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詳細情報 詳細情報について
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- CRID
- 1390001206293342336
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- NII論文ID
- 10008259295
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- NII書誌ID
- AN10453916
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- ISSN
- 13403494
- 18807291
- 13447882
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- NDL書誌ID
- 4846284
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- 本文言語コード
- ja
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- データソース種別
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- JaLC
- IRDB
- NDL
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- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可