Larger Loss of Activity in Taka-amylase A Modified with Starch-dialdehyde than with Dextran-dialdehyde.
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- Kobayashi Mikihiko
- National Food Research Institute
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- Tanaka Eiko
- National Food Research Institute
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- Takahara Hidenari
- Department of Resource Biology, Faculty of Agriculture, Ibaraki University
Bibliographic Information
- Other Title
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- ジアルデヒドー澱粉で修飾したタカアミラーゼAはジアルデヒドーデキストランで修飾したものより失活が大きい
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Abstract
Cross-linked modifications of Taka-amylase A (TAA) with various dialdehyde derivatives including starch- and dextran-dialdehyde were examined. A rapid and significant decrease in the amount of free amino groups and enzyme activity of TAA was attained by starch-dialdehyde, whereas dextran dialdehyde caused much lower loss in activity of TAA in spite of a similar extent of amino group modification. The molecular weight of the dextran-modified TAA increased by about 10, 000-20, 000 Da, which was measured by SDS-gel electrophoresis. A comparison of the activity of modified TAA on various substrates indicated a slight but definite increase in susceptibility to maltose and phenyl α-glucoside according to the increase in the extent of modification of TAA. In contrast, the activity of the modified TAA on maltotriose, maltotetraose, and γ-cyclodextrin decreased upon modification. Here, again, TAA modified with dextran-dialdehyde retained higher activity to those oligosaccharides than that modified with starch-dialdehyde. Moreover, inactivation caused by the fluorescent reagent, o-phthalaldehyde, was retarded by previous modification with dextran-dialdehyde, indicating that reaction between TAAand dialdehyde derivatives occurred through non-critical amino groups for enzyme activity.
Journal
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- Journal of Applied Glycoscience
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Journal of Applied Glycoscience 46 (4), 407-412, 1999
The Japanese Society of Applied Glycoscience
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Details 詳細情報について
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- CRID
- 1390282681269599360
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- NII Article ID
- 10008259352
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- NII Book ID
- AN10453916
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- ISSN
- 13403494
- 18807291
- 13447882
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- NDL BIB ID
- 4976515
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- Text Lang
- en
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- Data Source
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- JaLC
- IRDB
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed