1,5-アンヒドロフルクトースの調製と抗酸化活性 Preparation and Antioxidative Activity of 1,5-Anhydrofructose

Access this Article

Author(s)

Abstract

1,5-アンヒドロフルクトース(1,5-AF)の簡便な調製法を開発し,その抗酸化活性を調べた.まず,α-1,4-グルカンリアーゼ(EC4.2.2.13)を紅藻類のGracilaria verrucosa(おごのり)から抽出し,澱粉吸着により精製した.この酵素を用いて,360gのモチトウモPコシ澱粉からイオン交換樹脂とゲル濾過の行程を経て,150gの純度98.6%の1,5-AFを得た.産物は<SUP>I</SUP>H-<SUP>13</SUP>C COSY NMRによって1,5-AFと同定した.[α]<SUB>D</SUB><SUP>25</SUP>は-16.8°であった.1,5-AFの抗酸化力をリノール酸の酸化防止を対象として,アスコルビン酸(VC)と比較検討した.その結果,1,5-AFはVCに対して,チオバルビツーノレ酸法やチオアシン酸法では,ほぼ1.8倍の抗酸化力のあることが認められた.

A simple enzymic method for the preparation of 1, 5-anhydrofructose (1, 5-AF) and its antioxidative activity were investigated. α-1, 4-glucan lyase (EC 4 .2.2.13, GLase) was extracted and purified from red seaweed, Gracilaria verrrucosa. One-hundred-and-fifty grams of 1, 5-AF was obtained from 360 g of waxy maize starch by digestion with the purified GLase and the following purification of the product by ion-exchange resins and gel filtration. The purity of the product was 98 .6% by HPLC using an ionexchange resin (MC1 GEL CK08S). The product, 1, 5-AF, was identified by <SUP>I</SUP>H-<SUP>13</SUP>C COSY NMR, and the [α] <SUB>D</SUB><SUP>25</SUP> was -16.8°. The antioxidative activity of 1, 5-AF was examined by comparison with that of ascorbic acid (VC) for preventing the oxidation of linoleic acid . The antioxidative activity of 1, 5-AF was found to be approximately 1.8-fold that of VC by the methods using thiocyanate and 2-thiobarbituric acid.

Journal

  • Journal of Applied Glycoscience

    Journal of Applied Glycoscience 46(4), 439-444, 1999-12-01

    The Japanese Society of Applied Glycoscience

References:  15

Cited by:  6

Codes

  • NII Article ID (NAID)
    10008259440
  • NII NACSIS-CAT ID (NCID)
    AN10453916
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13403494
  • NDL Article ID
    4976626
  • NDL Source Classification
    ZP24(科学技術--化学・化学工業--糖・澱粉)
  • NDL Call No.
    Z17-15
  • Data Source
    CJP  CJPref  NDL  J-STAGE  JASI 
Page Top