アミノ基に特異的な試薬により修飾したタカアミラーゼAの失活 Inactivation of Taka-amylase A Modified by Chemical Reagents Specific to the Amino Groups

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Abstract

タカアミラーゼA(TAA)のアミノ基をトリニトロベンゼンスルホン酸,ホルムアルデヒド,ピリドキサール5'-りン酸で修飾し,蛍光試薬のオルトフタルアルデヒド(OPA)の場合と比較した.既報のように,OPAはTAAの強い失活をもたらすが,上記の3種類の試薬ではTAAの強い失活は起こらなかった三HPLCによる分析から,OPAとLys残基との反応はpHとCys残基の存在に依存して大きな差を生じた.これらの結果から,OPAとほかの3種の試薬によるTAAの修飾が異なる応答を示す理由として,Cys残基がOPA反応おいて重要な役割を果たしていることが示唆された.

The modification of amino groups in Taka-amylase A (TAA) using trinitrobenzene sulfonic acid (TNBS), formaldehyde, and pyridoxal 5'-phosphate (PLP) was compared with that using a fluorescent reagent, ο-phthalaldehyde (OPA). Although OPA resulted in potent inactivation of TAA as described in previous papers, the other three reagents described above caused no such inactivation of TAA. HPLC analysis showed that reactions of OPA with Lys residue differed greatly depending on the pH and the presence of Cys residue. Therefore, the different response of TAA for the modifications between OPA and other reagents suggested that Cys residue played an important role in the OPA reaction.

Journal

  • Journal of Applied Glycoscience

    Journal of Applied Glycoscience 46(4), 449-452, 1999-12-01

    The Japanese Society of Applied Glycoscience

References:  13

Cited by:  1

Codes

  • NII Article ID (NAID)
    10008259465
  • NII NACSIS-CAT ID (NCID)
    AN10453916
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13403494
  • NDL Article ID
    4976667
  • NDL Source Classification
    ZP24(科学技術--化学・化学工業--糖・澱粉)
  • NDL Call No.
    Z17-15
  • Data Source
    CJP  CJPref  NDL  J-STAGE  JASI 
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