コメ枝付け酵素RBE1の各種澱粉に対する反応の陰イオン交換-パルスアンペロメトリー高速クロマトグラフィー法による測定 Reaction of a Rice Branching Enzyme, RBE1, on Various Kinds of Starches Analyzed by High-Performance Anion Exchange Chromatography

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Abstract

米,馬鈴薯,甘藷,小麦,トウモロコシ,サゴ,タピオカ澱粉に,コメの枝付け酵素の一つであるRBE1を作用させ,陰イオン交換-パルスアンペロメトリー高速クロマトグラフィー(HPAEC-PAD)に供した.元の澱粉の構造に関わらず,RBE1処理された各種澱粉の構造は似通っており,10から11個のグルコース鎖の枝と,6個のグルコース鎖の枝の二つのピークを持つ形となった.RBE1反応の経時変化を調べたところ,長い糖鎖の転移は約10時間までに定常に達したが,短い6個の糖鎖は,反応22時間後でも増え続けた.アミロースでは短鎖,長鎖ともよく転移されたが,基質がすでに側鎖が多い場合には,RBE1による長鎖の転移が減少した.以上のことから,枝付け酵素の転移反応は,基質澱粉の側鎖の密度によって制限され,最終反応生成物の鎖長分布および側鎖の量はある定常状態に収束するものと考えられた.

The branch formations on starches from rice, potato, sweet potato, wheat, corn, sago, and tapioca using rice branching enzyme 1 (RBEI) were examined by high-performance anion exchange chromatography with a pulsed amperometric detector (HPAEC-PAD) after debranching with isoamylase. Irrespective of the difference of the structures of substrate starches, RBEI changed them to an almost similar structure with the two peaks at degrees of polymerization (DP) of 6 and 10 to 11 glucose chains. The longer chain transformation was saturated by 10-h incubation, while DP 6-glucose chain transformation was not saturated after 22-h incubation. When the structure of the substrate starch was not well branched, both DP 6-glucose chains and longer chains were well transferred by RBEI, but when the substrate was already well branched, the transfer reactions of longer chains decreased. The branching reaction seemed to be limited by the density of the glucose chains regardless of the structures of the original starches.

Journal

  • Journal of Applied Glycoscience

    Journal of Applied Glycoscience 46(4), 453-457, 1999-12-01

    The Japanese Society of Applied Glycoscience

References:  14

Cited by:  1

Codes

  • NII Article ID (NAID)
    10008259479
  • NII NACSIS-CAT ID (NCID)
    AN10453916
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13403494
  • NDL Article ID
    4976684
  • NDL Source Classification
    ZP24(科学技術--化学・化学工業--糖・澱粉)
  • NDL Call No.
    Z17-15
  • Data Source
    CJP  CJPref  NDL  J-STAGE  JASI 
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