Selective Isolation and Distribution of Actinomadura rugatobispora Strains in Soil

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Author(s)

Abstract

A simplified enrichment method for selective isolation of <I>Actinomadura rugatobispora</I> strains from soil is described. <I>A. rugatobispora</I> strains poorly produced spores on agar media when 5 to 20 colonies grew on a plate while characteristic spore formation was observed on humic acid-MOPS gellan gum medium. Spores of <I>A. rugatobispora</I> strains were tolerant to dry heating treatment at 120°C for 60 min. <I>A. rugatobispora</I> strains were resistant to 50 μg/ml trimethoprim and 10 μg/ml nalidixic acid, both of which inhibited the growth of nonfilamentous soil bacteria. In addition, these strains were more resistant to 100 μg/ml gentamicin and 20 μg/ml ampicillin than <I>Streptomyces</I> strains, which interfere with the growth of <I>A. rugatobispora</I> selectively. Owing these characteristics, we were successful to isolate 260 strains of this species from 148 out of 547 soil samples (27% of the samples tested). They were recovered from the soil samples with pH values ranging from 5.0 to 8.6, and 91% of the strains were isolated from neutral to slightly alkaline soil (pH 6.0 to 9.0). These results indicated that <I>A. rugatobispora</I> strains were distributed worldwide.

Journal

  • Actinomycetologica

    Actinomycetologica 14(2), 1-7, 2000-12-31

    The Society for Actinomycetes Japan

References:  32

Codes

  • NII Article ID (NAID)
    10008261022
  • NII NACSIS-CAT ID (NCID)
    AN10080091
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    09145818
  • NDL Article ID
    5662525
  • NDL Source Classification
    ZR5(科学技術--生物学--菌類・微生物)
  • NDL Call No.
    Z18-1070
  • Data Source
    CJP  NDL  J-STAGE 
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