Conjugative Transfer of Streptomyces Plasmid pSN22

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Abstract

pSN22, an 11 kb multicopy conjugative plasmid from <I>Streptomyces nigrifaciens</I>, promotes chromosome recombination in <I>Streptomyces lividans</I>. Five genes have been identified to be involved in plasmid transfer and pock formation: <I>traB</I> is essential for plasmid transfer; <I>traA</I> for pock formation; <I>spdA</I> and <I>spdB</I> are concerned with pock size; and <I>traR</I>, which corresponds to a <I>kor</I> gene in a <I>kil</I>-<I>kor</I> system, encodes a repressor of <I>traR</I> itself and the <I>traA</I>-<I>traB</I>-<I>spdB</I> (<I>tra</I>) operon. Studies on the interaction of TraR with promoter regions suggest that the negative regulation of transfer-related genes by TraR is achieved by two mechanisms, <I>i.e.</I> promoter hiding and roadblock. The predicted ATPase activity and the membrane localization of TraB suggest that the protein plays a direct role in ATP-driven DNA translocation. TraB is also thought to be involved in intra- and intermycelial transfers of pSN22.

Journal

  • Actinomycetologica

    Actinomycetologica 10(1), 12-22, 1996-06-15

    The Society for Actinomycetes Japan

References:  51

Codes

  • NII Article ID (NAID)
    10008262301
  • NII NACSIS-CAT ID (NCID)
    AN10080091
  • Text Lang
    ENG
  • Article Type
    REV
  • ISSN
    09145818
  • NDL Article ID
    3976990
  • NDL Source Classification
    ZR5(科学技術--生物学--菌類・微生物)
  • NDL Call No.
    Z18-1070
  • Data Source
    CJP  NDL  IR  J-STAGE 
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