Development of a Transformation System in Streptomyces virginiae.

• Kawachi Ryu

• Nihira Takuya

• Yamada Yasuhiro

書誌事項

タイトル別名

• Development of a transformation system

抄録

Streptomyces virginiae is one of the most difficult Streptomyces strains to transform because of its strong restriction barrier. We succeeded in transforming S. virginiae by combining heat treatment of protoplasts and in vitro methylation of plasmids. In order to avoid the host restriction barrier, plasmids were modified by methylase AluI, and S. virginiae protoplasts were treated at 42°C for 15 min prior to transformation. Under the optimal conditions, plasmids pIJ486, pKC1064 and pUWL-KS isolated from Streptomyces lividans were introduced into S. virginiae at a frequency of 1.4×10³, 7.0×10² and 1.2×10³ transformants per μg DNA, respectively. Plasmid pKC1064 and pUWL-KS isolated from Escherichia coli DH5α could not transform S. virginiae, but those isolated from E. coli JM110 (dam, dcm) could transform at a frequency of 1.0×10² and 2.4×10² transformants per μg DNA, indicating the presence of a methylation-specific restriction system in S. virginiae.

収録刊行物

• 日本放線菌学会誌

  日本放線菌学会誌 11 (2), 46-53, 1997

  日本放線菌学会

キーワード

• General Medicine

詳細情報

• CRID: 1390001204702697088
• NII論文ID: 130004487468
• NII論文ID: 10008262636
• NII書誌ID: AN10080091
• DOI: 10.3209/saj.11_46
• COI: 1:CAS:528:DyaK1cXjs1yrtbk%3D
• ISSN: 18816371
• ISSN: 09145818
• ISSN: http://id.crossref.org/issn/09145818
• NDL書誌ID: 4403977
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4403977
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可