Structures and Functional Analyses of Fungal Polyketide Synthase Genes

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Abstract

Polyketide synthase (PKS) genes have been cloned from several fungi, such as <I>Penicillium patulum MSAS</I>, <I>Aspergillus terreus atX</I> and <I>TPKS</I>, <I>Colletotrichum lagenarium PKS1</I>, <I>Aspergillus nidulans wA</I> and <I>stcA</I>, <I>Aspergillus parasiticus pksA</I>, <I>Cochliobolus heterostrophus CH-PKS1</I>. Their coded PKSs all belong to type I of multifunctional enzymes. In contrast to bacterial type I PKS of modular type, fungal PKSs are of iterative type and classified into three groups: A) single aromatic ring PKS, B) multi-aromatic ring PKS, and C) reduced complex-type PKS. To confirm actual function of fungal PKSs, <I>atX</I>, <I>PKS1</I>, and <I>wA</I> genes were expressed in heterologous fungi with the <I>amyB</I> controlled fungal expression system. Their product compounds were identified to be 6- methylsalicylic acid (1), 1,3,6,8-tetrahydroxynaphthalene (3), and naphthopyrone compound YWA1 (15), respectively. Modification of C-terminal region of WA PKS caused shift of product from YWA1 (15) to the same heptaketide compound citreoisocoumarin (14). This result indicated that domains previously designated as thioesterase of group B PKSs function as Claisen-type cyclase.

Journal

  • Actinomycetologica

    Actinomycetologica 12(1), 1-14, 1998-06-30

    The Society for Actinomycetes Japan

References:  69

Cited by:  1

Codes

  • NII Article ID (NAID)
    10008262732
  • NII NACSIS-CAT ID (NCID)
    AN10080091
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    09145818
  • NDL Article ID
    4524022
  • NDL Source Classification
    ZR5(科学技術--生物学--菌類・微生物)
  • NDL Call No.
    Z18-1070
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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