Development of a PCR/Southern dot blot based detection system for the presence of genes involved in beta-lactam biosynthesis

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Author(s)

Abstract

Screening of actinomycetes and fungi for genes involved in beta-lactam production can be carried out using a technique involving both PCR with conserved primers to the isopenicillin N synthase gene and a Southern dot blotting. This technique was successful with nine out of nine known beta-lactam producing Actinomycetes tested and three out of six known beta-lactam producing Fungi tested. It proved unable to detect the isopenicillin N synthase gene in the two <I>Flexibacter</I> beta lactam producing eubacteria strains tested. It also identified the presence of potential beta-lactam production genes in two known none beta-lactam producing actinomycetes and one out of fourteen novel newly isolated actinomycetes. This technique has potential for use in commercial screens for novel beta-lactam producers.

Journal

  • Actinomycetologica

    Actinomycetologica 12(1), 29-36, 1998-06-30

    The Society for Actinomycetes Japan

References:  20

Codes

  • NII Article ID (NAID)
    10008262828
  • NII NACSIS-CAT ID (NCID)
    AN10080091
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    09145818
  • NDL Article ID
    4524024
  • NDL Source Classification
    ZR5(科学技術--生物学--菌類・微生物)
  • NDL Call No.
    Z18-1070
  • Data Source
    CJP  NDL  J-STAGE 
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