Construction of Escherichia coli-Streptomyces Shuttle Vectors Transducible in Streptomyces

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Author(s)

Abstract

Shuttle vectors, pR4C10 and pR4C11, composed of <I>Streptomyces</I> cosmid and <I>Escherichia coli</I> plasmid were constructed. With both plasmids, <I>Streptomyces lividans</I> and <I>Escherichia coli</I> K12 strains were transformed at high frequencies, comparable with the original plasmids. A vector, pR4Cll, was shown to be encapsidated into particles of actinophage R4 <I>in vivo</I>, and transferred to <I>Streptomyces lividans</I>.

Journal

  • Actinomycetologica

    Actinomycetologica 12(1), 37-39, 1998-06-30

    The Society for Actinomycetes Japan

References:  11

Codes

  • NII Article ID (NAID)
    10008262849
  • NII NACSIS-CAT ID (NCID)
    AN10080091
  • Text Lang
    ENG
  • Article Type
    NOT
  • ISSN
    09145818
  • NDL Article ID
    4524025
  • NDL Source Classification
    ZR5(科学技術--生物学--菌類・微生物)
  • NDL Call No.
    Z18-1070
  • Data Source
    CJP  NDL  J-STAGE 
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