Gene Organization in a 12-Kilobase Region Covering afsA Encoding an A-Factor Biosynthetic Enzyme.
Bibliographic Information
- Other Title
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- Gene organization in a 12-kirobase region converting afsA encoding an A-factor biosynthetic enzyme
- Gene organization in a 12-kirobase regi
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Abstract
The nucleotide sequence of a 12-kilobase region covering afsA encoding a key enzyme for A- factor biosynthesis in Streptomyces griseus was determined and open reading frames were deduced by means of G+C plot analysis. Although this region about 150-kb from one end of the linear chromosome is known to be readily deleted, open reading frames crowd the region in a total of eleven, including afsA and sgaA involved in healthy growth on medium of high osmolality. These also include a pair of a sensor histidine kinase and a regulator which comprises a typical prokaryotic two-component regulatory system, proteins similar to O-methyltransferase, LysR-type regulator, 3-ketoacyl-acyl carrier protein reductase, and NADP-dependent alcohol dehydrogenase. In contrast to afsA-like genes in other Streptomyces strains, such as barX locating closely to the gene for the virginiae butanolide receptor in Streptomyces virginiae and farX locating closely to the IM-2-receptor gene in a Streptomyces sp., no open reading frames in this region in S. griseus show homology with the A-factor receptor protein, which suggests that the gene organization of receptor genes for γ-butyrolactone autoregulators differs from strain to strain.
Journal
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- Actinomycetologica
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Actinomycetologica 12 (1), 40-45, 1998
The Society for Actinomycetes Japan
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Details 詳細情報について
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- CRID
- 1390282679677942400
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- NII Article ID
- 10008262861
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- NII Book ID
- AN10080091
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- COI
- 1:CAS:528:DyaK1cXmtVagtrc%3D
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- ISSN
- 18816371
- 09145818
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- NDL BIB ID
- 4524026
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed