Multiple Domain Substitutions of Erythromycin Polyketide Synthase to Produce a Combinatorial Library.

• Thamchaipenet Arinthip

  Department of Genetics, Faculty of Science, Kasetsart University, Bangkhen Campus

抄録

The gene cluster encoding biosynthesis of the polyketide backbone of erythromycin is organised as repeated modules of functional domains. The similarity of the domain arrangements among the complex polyketide natural products provides a strategy to generate an array of different structures using a combinatorial approach. More than fifty 6-deoxyerythronolide B (6dEB) analogs were obtained by substitution of catalytic domains via single, double and triple alterations. This genetic manipulation approach to engineer such molecules, which would otherwise be impractical to produce by chemical methods, is very challenging and promising for the development of novel polyketides that could have utility in pharmaceutical applications in the future.

収録刊行物

• 日本放線菌学会誌

  日本放線菌学会誌 13 (2), 113-119, 1999

  日本放線菌学会

キーワード

• General Medicine

詳細情報

• CRID: 1390001204702822016
• NII論文ID: 10008263570
• NII書誌ID: AN10080091
• DOI: 10.3209/saj.13_113
• COI: 1:CAS:528:DC%2BD3cXht1alu7w%3D
• ISSN: 18816371
• ISSN: 09145818
• NDL書誌ID: 4948590
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4948590
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可