Multiple Domain Substitutions of Erythromycin Polyketide Synthase to Produce a Combinatorial Library

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Author(s)

Abstract

The gene cluster encoding biosynthesis of the polyketide backbone of erythromycin is organised as repeated modules of functional domains. The similarity of the domain arrangements among the complex polyketide natural products provides a strategy to generate an array of different structures using a combinatorial approach. More than fifty 6-deoxyerythronolide B (6dEB) analogs were obtained by substitution of catalytic domains <I>via</I> single, double and triple alterations. This genetic manipulation approach to engineer such molecules, which would otherwise be impractical to produce by chemical methods, is very challenging and promising for the development of novel polyketides that could have utility in pharmaceutical applications in the future.

Journal

  • Actinomycetologica

    Actinomycetologica 13(2), 113-119, 1999-12-31

    The Society for Actinomycetes Japan

References:  20

Codes

  • NII Article ID (NAID)
    10008263570
  • NII NACSIS-CAT ID (NCID)
    AN10080091
  • Text Lang
    ENG
  • Article Type
    REV
  • ISSN
    09145818
  • NDL Article ID
    4948590
  • NDL Source Classification
    ZR5(科学技術--生物学--菌類・微生物)
  • NDL Call No.
    Z18-1070
  • Data Source
    CJP  NDL  J-STAGE 
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