Determination of a novel bitter amino acid, pulcherrimine, in the gonad of the green sea urchin Hemicentrotus pulcherrimus.

DOI Web Site 被引用文献1件 参考文献19件
  • MURATA YUKO
    Marine Biochemistry Division, National Research Institute of Fisheries Science
  • SATA NORIKO U
    Marine Biochemistry Division, National Research Institute of Fisheries Science Faculty of Science and Technology, Keio University
  • YOKOYAMA MASAHITO
    Marine Biochemistry Division, National Research Institute of Fisheries Science Japan International Research Center for Agricultural Sciences
  • KUWAHARA RYUJI
    Marine Biochemistry Division, National Research Institute of Fisheries Science
  • KANENIWA MASAKI
    Marine Biochemistry Division, National Research Institute of Fisheries Science
  • OOHARA ICHIRO
    Marine Biochemistry Division, National Research Institute of Fisheries Science

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A novel bitter amino acid, pulcherrimine was analyzed by reverse-phase high-performance liquid chromatography using a linear gradient elution consisting of methanol/aqueous/acetic acid and methanol/acetic acid mixture, after being labeled with dimethylaminoazobenzenesulfonyl chloride (Dabs-Cl). Linearity of the calibration curve for pulcherrimine was high (r=0.994) in the tested range from 0 to 4 μg/mL. By this method, the pulcherrimine content in the ovary and testis of the green sea urchin Hemicentrotus pulcherrimus, collected in the sea off Iwaki in November 1998, was analyzed. The mean pulcherrimine content in the ovary was 1.37 mg/100 g. In contrast, no pulcherrimine was detected in all testes examined. Correlation between the bitterness and the pulcherrimine content was statistically significant. These results strongly support that pulcherrimine is a bitter principle in the green sea urchin ovaries.

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