Comparison of the amino acid sequences of acorn barnacle lectins showing different inhibitory activities toward the crystal growth of calcium carbonate.

• MURAMOTO KOJI

  Department of Biological Resource Sciences, Graduate School of Agricultural Science, Tohoku University

• JIN DONG-HAO

  Department of Biological Resource Sciences, Graduate School of Agricultural Science, Tohoku University

• NIINO YOKO

  Department of Biological Resource Sciences, Graduate School of Agricultural Science, Tohoku University

• FUJIWARA KAZUE

  Department of Biological Resource Sciences, Graduate School of Agricultural Science, Tohoku University

• KABUTO SHIZUYA

  Department of Biological Resource Sciences, Graduate School of Agricultural Science, Tohoku University

• OGAWA TOMOHISA

  Department of Biological Resource Sciences, Graduate School of Agricultural Science, Tohoku University

• TODA MICHITOSHI

  Department of Marine Biochemistry, School of Fisheries Sciences, Kitasato University

• KAMIYA HISAO

  Department of Marine Biochemistry, School of Fisheries Sciences, Kitasato University

抄録

The amino acid sequence of a D-galactose-binding lectin isolated from the hemolymph of the acorn barnacle Balanus rostratus was determined. The lectin (BRL) (M_r 120K) is a multimeric protein whose subunit consists of 182 amino acids. The amino acid sequence was compared with those of multiple lectins (BRA-2, BRA-3) from Megabalanus rosa to explore the relationship between the structures and the inhibitory activity toward the crystal growth of calcium carbonate. Although BRL was 46% identical to BRA-2 and 15% identical to BRA-3, the lectin had no inhibitory activity, unlike BRA-2 and BRA-3. Both the number and the localization of acidic amino acid residues and their amide forms were different among them. Observations by scanning electron microscopy revealed modifications in the size and morphology of the calcium carbonate crystals grown in the presence of the lectins.

収録刊行物

• Ｆｉｓｈｅｒｉｅｓ　ｓｃｉｅｎｃｅ

  Ｆｉｓｈｅｒｉｅｓ　ｓｃｉｅｎｃｅ 67 (4), 703-709, 2001

  公益社団法人 日本水産学会

キーワード

• acorn barnacle
• agglutinin
• amino acid sequence
• Balanus rostratus
• hemagglutinin
• hemolymph
• lectin

詳細情報

• CRID: 1390282679406073344
• NII論文ID: 130003903605
• NII論文ID: 10008270119
• NII書誌ID: AA10993718
• DOI: 10.1046/j.1444-2906.2001.00309.x
• COI: 1:CAS:528:DC%2BD3MXmsFyntb4%3D
• ISSN: 14442906
• ISSN: 09199268
• NDL書誌ID: 5893086
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5893086
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可