Random amplified polymorphic DNA markers for three Japanese laminarian species.

• YOTSUKURA NORISHIGE

  Institute of Algological Research, Faculty of Science, Hokkaido University

• KAWAI TADASHI

  Hokkaido Fisheries Experimental Station

• MOTOMURA TAIZO

  Institute of Algological Research, Faculty of Science, Hokkaido University

• ICHIMURA TERUNOBU

  Institute of Algological Research, Faculty of Science, Hokkaido University

抄録

Random amplified polymorphic DNA (RAPD) was studied to detect genetic markers for three economically important Japanese laminarian species, Laminaria japonica Areschoug, L. religiosa Miyabe and L. ochotensis Miyabe, which were sampled from their representative localities on the coasts from Tsugaru Straits to the Sea of Japan. DNA templates for RAPD were extracted from lamina using a DNA extraction kit and were purified with glassmilk. Reproducible RAPD markers for these three species were detected using three random primers from a total of 15 tested. In L. japonica and L. religiosa, these RAPD markers were confirmed to be useful for populations from other localities. The three species studied showed high intraspecific and interspecific band sharing index (BSI) values. Like annual typical L. religiosa from other localities, biennial individuals identified as putative L. religiosa from Atsuta could be discriminated from L. japonica but not from L. ochotensis by any of the RAPD genetic markers studied so far.

収録刊行物

• Ｆｉｓｈｅｒｉｅｓ　ｓｃｉｅｎｃｅ

  Ｆｉｓｈｅｒｉｅｓ　ｓｃｉｅｎｃｅ 67 (5), 857-862, 2001

  公益社団法人 日本水産学会

キーワード

• genetic marker
• Japanese kelp
• Laminaria
• random amplified polymorphic DNA
• species identification

詳細情報

• CRID: 1390282679404457344
• NII論文ID: 130003742373
• NII論文ID: 10008270639
• NII書誌ID: AA10993718
• DOI: 10.1046/j.1444-2906.2001.00333.x
• ISSN: 14442906
• ISSN: 09199268
• NDL書誌ID: 5950824
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5950824
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可