Interaction of grammistins with lipids and their antibacterial activity.
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- YOKOTA HIROSHI
- Department of Food Science and Technology, Tokyo University of Fisheries
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- NAGASHIMA YUJI
- Department of Food Science and Technology, Tokyo University of Fisheries
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- SHIOMI KAZUO
- Department of Food Science and Technology, Tokyo University of Fisheries
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抄録
Grammistins are hemolytic and ichthyotoxic peptides in the skin secretion of soapfishes and are structurally characterized by their abundance in amphiphilic α-helicity. In the present study, their interaction with lipids and lipid vesicles as well as antibacterial activity were examined using four grammistins (Gs 1 and Gs 2 from Grammistes sexlineatus and Pp 1 and Pp 3 from Pogonoperca punctata). The hemolytic activity of grammistins was inhibited by phospholipids but not by cholesterol. Moreover, grammistins released carboxyfluorescein entrapped within liposomes made of phosphatidylcholine. In contrast, grammistins were found to have antibacterial activity with a broad spectrum against nine species of bacteria, including both Gram-negative and Gram-positive groups. The potency of their antibacterial activity was not related to that of hemolytic activity, suggesting that grammistins bind to membrane phospholipids but lyse erythrocyte and bacterial membranes via different mechanisms. Conclusively, grammistins are new members of the family of cell non-selective membrane-lytic peptides with amphiphilic α-helices, being similar to pardaxins, which are secreted from the skin of soles, and to melittin, which is derived from bee venom.
収録刊行物
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- Fisheries science
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Fisheries science 67 (5), 928-933, 2001
公益社団法人 日本水産学会
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詳細情報 詳細情報について
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- CRID
- 1390001204427766400
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- NII論文ID
- 130003742383
- 10008270878
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- NII書誌ID
- AA10993718
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- ISSN
- 14442906
- 09199268
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- NDL書誌ID
- 5951007
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
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