Effects of urea and trimethylamine-N-oxide on ATPase of requiem shark myofibril and its constituents.
-
- KANOH SATOSHI
- Faculty of Bioresources, Mie University
-
- KITAMURA MASAYA
- Faculty of Bioresources, Mie University
-
- HORIE YUTA
- Faculty of Bioresources, Mie University
-
- KASAMA YUUKI
- Faculty of Bioresources, Mie University
-
- NIWA EIJI
- Faculty of Bioresources, Mie University
この論文をさがす
抄録
The effects of trimethylamine-N-oxide (TMAO) on the urea-resistibility of requiem shark myofibrils were investigated, using Ca2+- and Mg2+-ATPase activities as a parameter. Both activities were hardly changed or activated up to 0.6 M urea. In contrast, the two activities both decreased to less than 50% in the presence of TMAO up to 0.5 M. When measured at a 2 : 1 molar ratio of urea and TMAO, Ca2+- and Mg2+-ATPase activities were similar to those in the presence of TMAO alone, indicating that TMAO reduced the urea-resistibility of myofibrils. Myosin, the most abundant protein in myofibrils, from requiem shark exhibited the effects of urea and TMAO on its Ca2+-ATPase activity, which was primarily similar to those of myofibrils. However, Ca2+-ATPase activities in the coexistence of urea and TMAO for actomyosin reconstituted from requiem shark myosin and chicken F-actin were approximately average of those measured independently in the presence of either urea or TMAO alone. Carp myofibrils, reconstituted actomyosin and myosin, which were used as teleost references, all showed a tendency in the effects of urea and TMAO on Ca2+-ATPase activities that was similar to those of requiem shark counterparts.
収録刊行物
-
- Fisheries science
-
Fisheries science 67 (5), 943-947, 2001
公益社団法人 日本水産学会
- Tweet
キーワード
詳細情報 詳細情報について
-
- CRID
- 1390001204427764352
-
- NII論文ID
- 130003742385
- 30014677465
- 10008270917
-
- NII書誌ID
- AA10993718
-
- COI
- 1:CAS:528:DC%2BD3MXosV2hsbs%3D
-
- ISSN
- 14442906
- 09199268
-
- NDL書誌ID
- 5951046
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可