Isolation and characterization of the interspecific fusants from Streptomycetes obtained using a liquid regeneration method

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Abstract

Protoplast fusion between different species of <i>Streptomyces</i> was performed using a liquid regeneration method developed for a rapid and simple preparation of the fusants. Consequently, new clones, which could not be obtained using the conventional agar regeneration method, were obtained. In the crosses between <i>S</i>. <i>griseus</i> and <i>S</i>. <i>durhamensis</i>, and between <i>S</i>. <i>californicus</i> and <i>S</i>. <i>catenulae</i>, eight and two recombinants, respectively, were obtained using the liquid regeneration method. Conversely, in the case of crosses between <i>S</i>. <i>ornatus</i> and <i>S</i>. <i>catenulae</i>, and between <i>S</i>. <i>ornatus</i> and <i>S</i>. <i>vendargensis</i>, seven recombinants each were obtained using only the agar method. The physiological characteristics, such as the assimilation of carbohydrate and antibiotic resistance, of these fusants differed considerably from those of their parental strains. Using the proposed liquid regeneration method, a simpler and quicker procedure for protoplast fusion is described.

Journal

  • Fisheries science

    Fisheries science 68(2), 395-402, 2002-04

    The Japanese Society of Fisheries Science

References:  10

Cited by:  1

  • Protoplast fusion between marine bacterium and Escherichia coli with reference to the distribution of parental characteristics in fusants  [in Japanese]

    ZHANG ENSHI , IMADA CHIAKI , KAMATA MASAZUMI , KOBAYASHI TAKESHI , HAMADA SATO NAOKO , ENSHI ZHANG , CHIAKI IMADA , MASAZUMI KAMATA , TAKESHI KOBAYASHI , NAOKO HAMADA-SATO , 東京海洋大学大学院海洋科学技術研究科 , 東京海洋大学大学院海洋科学技術研究科 , 山野美容芸術短期大学美容芸術学科 , 東京海洋大学大学院海洋科学技術研究科 , 東京海洋大学大学院海洋科学技術研究科 , Graduate School of Marine Science and Technology Tokyo University of Marine Science and Technology , Graduate School of Marine Science and Technology Tokyo University of Marine Science and Technology , Science of Aesthetics and Art Yamano College of Aesthetics , Graduate School of Marine Science and Technology Tokyo University of Marine Science and Technology , Graduate School of Marine Science and Technology Tokyo University of Marine Science and Technology

    NIPPON SUISAN GAKKAISHI 72(4), 743-745, 2006-07-15

    J-STAGE  JASI  References (16) Cited by (2)

Codes

  • NII Article ID (NAID)
    10008273357
  • NII NACSIS-CAT ID (NCID)
    AA10993718
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    09199268
  • NDL Article ID
    6152088
  • NDL Source Classification
    ZR26(科学技術--農林水産--水産)
  • NDL Call No.
    Z54-H592
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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