硫酸鉄とアスコルビン酸によって脂質過酸化誘起されたブタ精子の15℃液状保存後の精子生存指数  [in Japanese] Viability after Liquid Storage at 15℃ of Boar Sperm Induced Lipid Peroxidation  [in Japanese]

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Abstract

硫酸鉄 (FeSO<sub>4</sub>) とビタミンC (Ascorbic acid: VC) により脂質過酸化誘起したブタ精子の15℃液状保存後の精子生存指数を調べた。さらに Hypotaurine (HT) を脂質過酸化誘起処置液に添加し, その後の液状保存後の精子生存指数も調べた。11頭の種雄豚より採取した精液の精漿を除去して, 脂質過酸化誘起処置液で精子数を3.0×10<sup>8</sup>/m<i>l</i>に希釈し, 38℃で1時間インキュベートすることによって脂質過酸化誘起処置をおこなった。この精子脂質過酸化誘起処置液は, VC 0.5mMとFeSO<sub>4</sub>を0.01, 0.1もしくは1mM含む Modena を用いた。そして, 脂質過酸化誘起処置直後の Malonaldehyde (MDA) 生成量を分析した。これらの脂質過酸化誘起処置精子を15℃で液状保存し, 精子生存指数を処置直後, 保存後48, 96, 144, 192, 240および288時間目に調べた。また, FeSO<sub>4</sub> 0.1mMを含む脂質過酸化処置液にHTを50mM添加して脂質過酸化誘起処置をおこない, 処置直後のMDA生成量および精子生存指数を調べた。さらに, この誘起処置精子を15℃液状保存し, その精子生存指数を処置直後, 保存後48, 96, 144, 192, 240および288時間目に調べた。0.1もしくは1mMのFeSO<sub>4</sub>とVCによる脂質過酸化誘起処置によりMDA生成量が有意に増加した (P<0.001)。これらの脂質過酸化誘起処置精子の15℃液状保存後の精子生存指数は, 保存48時間目以降および処置直後から, 無処置精子よりも有意に低下した (P<0.05)。HT 50mMを含む脂質過酸化誘起処置においては, その精子生存指数は無処置精子と比較して有意な低下が認められなかった。しかし, HT 50mMを含む処置精子のMDA生成量はHT 50mMを含まない処置精子のものと比較し有意な差は認あられなかった。以上のことから, ブタ精液15℃液状保存前に脂質過酸化が生じた精子は, 保存後の精子生存指数の低下が認められた。また, 15℃液状保存前のFeSO<sub>4</sub> 0.1mM脂質過酸化誘起処置液への50mMのHT添加は, 15℃液状保存後の精子生存指数の低下を抑制することが明らかになった。

We examined the viability of boar sperm, after liquid storage at 15°C, in which lipid peroxidation had occurred. Also the viability of sperm treated with ferrus sulfate, Ascorbic acid (VC) and hypotaurine (HT) were investigated. Boar semen collected from 11 boars was centrifuged and diluted with lipid peroxidation induction media which were made of Modena including 0.01, 0.1, 1mM ferrus sulfate and 0.5mM VC. The sperm concentration of the diluted semen was 3.0×10<sup>8</sup>/m<i>l</i>. Then the media were incubated at 37°C in air for 1 hour. Lipid peroxidation of sperm incubated with ferrus sulfate and VC was estimated by malonaldehyde (MDA) concentration. The incubated semen was stored at 15°C for up to 288 hours and the viability of sperm was examined. Additionally, MDA was measured in semen incubated with ferrus sulfate, VC and HT and incubated semen was stored at 15°C for up to 288 hours. Then the viability of the stored semen was also determined. The amount of generated MDA from sperm incubated with 0.1 or 1mM ferrus sulfate was significantly over sperm in which lipid peroxidation was not induced (P<0.001). After liquid storage for 48 hours, the viability of sperm induced lipid peroxidation significantly lower than that of non treated sperm (P<0.05). The viability of sperm treated with ferrus sulfate and HT was not significantly different compared with non treated sperm. However, the level of MDA from sperm incubated with HT was not significantly lower than sperm incubated without HT. These data indicate that lipid peroxidation occurred before liquid storage at 15°C and the viability of boar sperm decreases after storage. Thus the addition of HT into the medium to induce sperm lipid peroxidation would improve the viability of sperm after liquid storage at 15°C.

Journal

  • Nihon Yoton Gakkaishi

    Nihon Yoton Gakkaishi 37(1), 16-22, 2000-03-10

    The Japanese Society of Swine Science

References:  25

Codes

  • NII Article ID (NAID)
    10008283984
  • NII NACSIS-CAT ID (NCID)
    AN10202971
  • Text Lang
    JPN
  • Article Type
    ART
  • ISSN
    0913882X
  • NDL Article ID
    5298488
  • NDL Source Classification
    ZR22(科学技術--農林水産--畜産)
  • NDL Call No.
    Z18-1082
  • Data Source
    CJP  NDL  J-STAGE  JASI 
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