Effects of Reactive Oxygen Species and Antioxidants on the Motility of Boar Spermatozoa in the Process of Freezing and Thawing.

DOI JASI Web Site 1 Citations 26 References

Bibliographic Information

Other Title
  • 凍結・融解過程における活性酸素種および抗酸化剤がブタ精子の活力に及ぼす影響
  • トウケツ ユウカイ カテイ ニ オケル カッセイ サンソシュ オヨビ コウサンカザイ ガ ブタ セイシ ノ カツリョク ニ オヨボス エイキョウ

Search this article

Abstract

The objective of this study was to examine the effects of reactive oxygen species (ROS) produced by a xanthine (X)-xanthine oxidase (XO) generating system on the motility of boar spermatozoa in the process of freezing-thawing, and to evaluate the ability of the antioxidants to protect sperm from this challenge.<br>The sperm rich fraction was diluted with the pre-diluent and stored for 3hrs at room temperature, and then divided into 6 tubes before centrifugation. Precipitated sperm in each tube was suspended in one of the following lactose-egg yolk diluents added antioxidants. 1) Lactose-egg yolk (control), 2) X (0.6mM)-XO (0.05U/ml), 3) X-XO and catalase (Cat) (150U/ml), 4) X-XO and super oxide dismutase (SOD) (150U/ML), 5) X-XO and glutathione (GSSG) (1.5mM) or 6) X-XO and hypotaurine (HT) (10mM). Each group of sperm was cooled to 5°C and then added the 2nd diluent including glycerol and OEP. Finally diluted semen was packaged in the maxi-straw and then frozen in liquid nitrogen vapor. It took about 2hrs from the exposure of ROS to freeze.<br>After the semen was thawed at 45°C, it was diluted with the thawing-diluent and incubated at 37°C for 60 minutes to observe the motility. The results were expressed as the mean±S. E. of 9 ejaculates from 3 boars.<br>Frozen-thawed sperm with X-XO alone was significantly low in revival rate in incubation compared to the control group. The revival rate of the X-XO and catalase group in incubation was significantly high compared to that of the X-XO alone group. Also high revival rates were obtained in both groups of X-XO and SOD, and X-XO and hypotaurine after incubation showed significantly high values in the sperm with X-XO and catalase, and with X-XO and hypotaurine compared to that of the X-XO alone group.<br>These results indicate that ROS adversely affect motility in the process of freezing and thawing of boar sperm. Catalase completely inhibited the decline of motility in the presence of X-XO indicating that hydrogen peroxide is the primary ROS affecting sperm motility. Hypotaurine is also valuable to inhibit adverse effect of ROS.

Journal

Citations (1)*help

See more

References(26)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top