Viability after Liquid Storage at 15.DEG.C. of Boar Sperm Induced Lipid Peroxidation.

• NAKATSUKASA Ena

  School of Veterinary Medicine, Azabu University

• KASHIWAZAKI Naomi

  School of Veterinary Medicine, Azabu University

• SHINO Masao

  School of Veterinary Medicine, Azabu University

Bibliographic Information

Other Title

• 硫酸鉄とアスコルビン酸によって脂質過酸化誘起されたブタ精子の１５℃液状保存後の精子生存指数
• リュウサンテツ ト アスコルビンサン ニ ヨッテ シシツ カサンカ ユウキ サレタ ブタ セイシ ノ 15ドC エキジョウ ホゾン ゴ ノ セイシ セイゾン シスウ

Abstract

We examined the viability of boar sperm, after liquid storage at 15°C, in which lipid peroxidation had occurred. Also the viability of sperm treated with ferrus sulfate, Ascorbic acid (VC) and hypotaurine (HT) were investigated. Boar semen collected from 11 boars was centrifuged and diluted with lipid peroxidation induction media which were made of Modena including 0.01, 0.1, 1mM ferrus sulfate and 0.5mM VC. The sperm concentration of the diluted semen was 3.0×10⁸/ml. Then the media were incubated at 37°C in air for 1 hour. Lipid peroxidation of sperm incubated with ferrus sulfate and VC was estimated by malonaldehyde (MDA) concentration. The incubated semen was stored at 15°C for up to 288 hours and the viability of sperm was examined. Additionally, MDA was measured in semen incubated with ferrus sulfate, VC and HT and incubated semen was stored at 15°C for up to 288 hours. Then the viability of the stored semen was also determined. The amount of generated MDA from sperm incubated with 0.1 or 1mM ferrus sulfate was significantly over sperm in which lipid peroxidation was not induced (P<0.001). After liquid storage for 48 hours, the viability of sperm induced lipid peroxidation significantly lower than that of non treated sperm (P<0.05). The viability of sperm treated with ferrus sulfate and HT was not significantly different compared with non treated sperm. However, the level of MDA from sperm incubated with HT was not significantly lower than sperm incubated without HT. These data indicate that lipid peroxidation occurred before liquid storage at 15°C and the viability of boar sperm decreases after storage. Thus the addition of HT into the medium to induce sperm lipid peroxidation would improve the viability of sperm after liquid storage at 15°C.

Journal

• Nihon Yoton Gakkaishi

  Nihon Yoton Gakkaishi 37 (1), 16-22, 2000

  The Japanese Society of Swine Science

Keywords

• boar
• semen
• lipid peroxidation
• viability of sperm
• liquid storage

Details

• CRID: 1390001204706679040
• NII Article ID: 10008283984
• NII Article ID: 10008284215
• NII Book ID: AN10202971
• DOI: 10.5938/youton.37.16
• ISSN: 1881655X
• ISSN: 0913882X
• NDL BIB ID: 5298488
• Web Site: https://agriknowledge.affrc.go.jp/RN/2010602395
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5298488
• Text Lang: ja
• Data Source:
  • JaLC
  • IRDB
  • NDL
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed