未成熟豚における排卵誘起時の性ステロイドおよびプロスタグランジンの動態  [in Japanese] Changes of sex steroids and Prostaglandins around induced ovulation  [in Japanese]

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Abstract

排卵に伴う各種ステロイドおよびPGの動態について明らかにするたあに, 48頭の未成熟豚に, PMSG 12IU/kg体重を筋肉内注射した。72時間後にhCG 6IU/kg体重を筋肉内注射し, hCG投与後経時的に開腹し, 排卵の状況を検査し, あわせて末梢血中, 卵巣静脈血中及び卵胞液中の性ステロイド値及びPGの動態を検査した。<br>その結果, 排卵率は, hCG投与後24時間までは0%で, 32時間後16%, 36時間後60%, 42時間後80%, 48時間後86%, 72時間後85%であった。したがって, 排卵はhCG投与後32~48時間の間に起こるものと推察された。末梢血中, 卵巣静脈血中および卵胞液中の性ステロイドについては, Estradiol-17βおよび testosterone 値は排卵がちかずくにつれて低下し, Progesterone 値は排卵後著明に増加した。また, 卵巣静脈血中PGF<sub>2α</sub>は排卵がちかずくにつれて増加した。さらに, 卵胞液中PGE<sub>1</sub>, PGF<sub>2α</sub>および6-keto-PGF<sub>1α</sub>も排卵がちかずくにつれてそれぞれ増加した。<br>以上の結果より, 排卵時に卵巣においてPG類が産生されることが明らかとなり, 排卵にPGが関係することが示唆された。

The present study was conducted to clarify the changes of sex steroids and Prostaglandins around induced ovulation in the prepubertal gilts.<br>Forty eight prepubertal gilts were injected intramuscularly with hCG (6IU/kg of body weight; kg.b.w.) 72 hours after receiving PMSG (12IU/kg.b.w.). Ovulation was verified by laparotomy. Changes in concentrations of steroids and Prostaglandins in peripheralblood plasma, ovarian vein blood plasma and follicular fluid were examined.<br>Ovulation rate was 0% until 24 hours after hCG injection and 16%, 60%, 80%, 86%, 85% at 32, 36, 42, 48, 72 hours after hCG injection, respectively. And it is suggested that ovulation occurred 32 to 48 hours after hCG injection. Regarding the levels of sex steroids in the pheripheral blood plasma, ovarian vein blood plasma and follicular fluid, estradiol-17β and testosterone levels decreased as the time of ovulation approached. Progesterone levels increased markedly after ovulation. PGF<sub>2α</sub> levels in the ovarian vein blood plasma increased near the time of ovulation. Furthermore, the levels of PGE<sub>1</sub>, PGF<sub>2α</sub> and 6-keto-PGF<sub>1α</sub> in the follicular fluid also increased rapidly near the time of ovulation.<br>Based on these results, it is suggested that PGs are produced in the ovaries as the time of ovulation approaches and that PGs have some roles on ovulation.

Journal

  • Nihon Yoton Gakkaishi

    Nihon Yoton Gakkaishi 34(4), 157-162, 1997-12-25

    The Japanese Society of Swine Science

References:  21

Codes

  • NII Article ID (NAID)
    10008284821
  • NII NACSIS-CAT ID (NCID)
    AN10202971
  • Text Lang
    JPN
  • Article Type
    ART
  • ISSN
    0913882X
  • NDL Article ID
    4381812
  • NDL Source Classification
    ZR22(科学技術--農林水産--畜産)
  • NDL Call No.
    Z18-1082
  • Data Source
    CJP  NDL  J-STAGE  JASI 
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