家畜肉から抽出した筋漿タンパク質のHPLC分画パターンによる肉種の判別  [in Japanese] Identification of Meat Species by HPLC Patterns of Sarcoplasmic Protein Extracted from Acetone-dried Muscle Powder of Cattle, Horse, Pig, Goat and Sheep  [in Japanese]

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Author(s)

    • 松岡 昭善 MATSUOKA Akiyoshi
    • 東京農業大学農学部畜産物利用学研究室 Laboratory of Utilization of Animal Products, Department of Zootechnical science, Tokyo University of Agriculture
    • 古川 徳 FURUKAWA Noboru
    • 東京農業大学農学部畜産物利用学研究室 Laboratory of Utilization of Animal Products, Department of Zootechnical science, Tokyo University of Agriculture
    • 高橋 強 [他] TAKAHASHI Tsuyoshi
    • 東京農業大学農学部畜産物利用学研究室 Laboratory of Utilization of Animal Products, Department of Zootechnical science, Tokyo University of Agriculture
    • 山中 良忠 YAMANAKA Yoshitada
    • 東京農業大学農学部畜産物利用学研究室 Laboratory of Utilization of Animal Products, Department of Zootechnical science, Tokyo University of Agriculture

Abstract

細切した牛, 馬, 豚, ヤギおよびヒッジの筋肉を-10℃の冷アセトンで脱脂, 粉末化した試料 (アセトン粉末) から筋漿タンパク質を抽出し, 逆相HPLCを用いて分画を行い, 分面パターンによる肉種の識別の可能性を検討した. その結果, 本実験に用いた方法で筋漿タンパク質を抽出することによって, 分画されたピークの相対保持時間 (RRt) は再現精度が向上し, カラム圧の上昇を遅延させる効果が認められた. ピーク面積は, 同一種においても個体間で比較的変動が大きい傾向が認あられた.<br>牛, 馬, 豚, ヤギおよびヒツジの筋漿タンパク質のクロマトグラムには, 畜種固有のピークあるいはピークパターンが認あられ, これにより5畜種の識別が可能と考えられた. さらに, 得られたクロマトグラムの相対保持時間に基づいてピークを10区分に分け, 各区分のピーク面積を判別因子として判別分析を行った結果, 牛では92.3%, 馬では94.1%, 豚では100%, ヤギでは88.9%, ヒツジでは100%の正判別率を示す判別式が得られた.<br>以上の結果から, 筋漿タンパク質のHPLCクロマトグラムにおけるピークパターンおよび相対保持時間により区分けしたピーク面積を判別因子とする判別分析を併用することにより, 本実験で対象とした5畜種をより正確に識別することが可能と考えられた.

The possibility of identification of meat species by HPLC patterns of sarcoplasmic protein was investigated. In this study, sarcoplasmic proteins extracted from <i>M. longissimus thoracis</i> of cattle, horse, pig, goat and sheep which were treated with acetone cooled below -10°C (acetone-dried muscle powder) in order to eliminate the muscle lipid were analyzed by reverse-phase HPLC. The HPLC patterns of the sarcoplasmic protein obtained were then statistically analyzed by discriminant analysis.<br>The reliability of RRt was improved significantly by using sarcoplasmic proteins extracted from acetone-dried muscle powder. There was a tendency that peak area of the protein fractions was different between individuals in a species.<br>The chromatograms of the sarcoplasmic proteins from cattle, horse, pig, goat and sheep revealed the patterns specific to the species, respectively, and suggested that the five species can be distinguished one from another.<br>Results of discriminant analysis showed that the classification rule identified 12 in 13 samples for cattle (92.3%), 16 in 17 samples for horse (94.1%), 25 in 25 samples for pig (100%), 8 in 9 samples for goat (88.9%), and 10 in 10 samples for sheep (100%).

Journal

  • Nihon Yoton Gakkaishi

    Nihon Yoton Gakkaishi 34(4), 163-169, 1997-12-25

    The Japanese Society of Swine Science

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Codes

  • NII Article ID (NAID)
    10008284843
  • NII NACSIS-CAT ID (NCID)
    AN10202971
  • Text Lang
    JPN
  • Article Type
    ART
  • ISSN
    0913882X
  • NDL Article ID
    4381813
  • NDL Source Classification
    ZR22(科学技術--農林水産--畜産)
  • NDL Call No.
    Z18-1082
  • Data Source
    CJP  NDL  J-STAGE  JASI 
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