子豚の群編成と末梢血コルチゾール濃度,リンパ球幼若化能,単球貪食能およびフィトヘマグルチニン(PHA)皮内反応  [in Japanese] Effects of Grouping of Piglets on Plasma Cortisol Concentration, Lymphocyte Blastogenesis and Phagocytotic Function of Monocytes in Peripheral Blood and on Intradermal Response to Pytohemagglutinin  [in Japanese]

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Author(s)

    • 出口 栄三郎 DEGUCHI Eisaburo
    • 鹿児島大学農学部獣医学科家畜内科学講座 Laboratory of Veterinary Internal Medicine, Department of Veterinary Medicine, Faculty of Agriculture, Kagoshima University

Abstract

本試験は, 子豚の群編成が血漿コルチゾール濃度, 白血球数, 白血球百分比, 単球の貪食能, リンパ球機能に及ぼす影響を明らかにする目的で実施した。LW種去勢雄のSPF子豚, 4頭/腹および8頭/腹の計12頭を用いた。生後63.0日齢に, 8頭/腹のうち4頭の子豚を, 別の1腹4頭を飼育している豚房に移動し, 13日間同居させた。移動した子豚を移動子豚, また, 移動せずに編成した子豚を先住子豚とした。残りの1腹8頭のうちの4頭は, 編成せずに対照子豚とした。編成直後から2時間, 先住子豚は移動子豚に対して, 激しく耳を噛むあるいは前肢で肩に乗るなどの攻撃がみられた。その後, 子豚間の闘争は試験終了まで全くみられなかった。編成後2時間における血漿コルチゾール濃度は, 編成直前に比較して有意に高く, 編成後24時間には編成直前の値まで低下した。試験期間中, 白血球数と白血球百分比には有意な変動は認められなかった。先住子豚の編成後3日における, ポークウィードマイトジェン (PWM) およびフィトヘマグルチニン (PHA) により誘発されたリンパ球幼若化能 (<i>in vitro</i> リンパ球機能検査) は, 編成前3日に比較して有意に低下していた。先住子豚および移動子豚における, 単球の酵母貪食能およびPHA皮内反応 (<i>in vivo</i> リンパ球機能検査) は, 編成前3日に比較して編成後13日まで有意に低下していた。対照子豚でも, 血漿コルチゾール濃度が軽度上昇し, 編成後3日に一時的な単球貪食能の低下がみられたが, PHA皮内反応は編成後13日でも低下していた。<br>これらの成績から, 群編成は子豚にとってストレスであり, 編成後少なくとも13日間, リンパ球機能と単球貪食能は抑制されていることが示された。

This experiment was carried out to reexamine the effects of grouping of piglets on the concentration of plasma cortisol, total and differential leukocyte counts, the phagocytic functions of monocytes and the function of lymphocytes <i>in vivo</i> and <i>in vitro</i>. Twelve specific-pathogen-free piglets from 8 piglets/litter and 4 piglets/litter were used. At 63 days of age, 4 piglets of 8 piglets of a litter (Group A) were moved to the pen where 4 piglets of the other litter (Group B) were housed, and they were reared together for 13 days. The remaining 4 piglets of 8 piglets were not moved but reared for 13 days as control piglets. After grouping, all piglets of Group B attacked violently the piglets of Group A and bited their shoulders and ears for 2 hours. The concentrations of plasma cortisol at 2 hours after grouping were significantly higher as compared with those on 3rd day before grouping. After then, the plasma cortisol concentrations dropped to the normal and base-line levels at 24 hours after grouping. A significant difference in plasma cortisol concentration was not observed between Groups A and B. The numbers of total and differential leukocytes were almost same throughout the experiment. The proliferative responses of peripheral blood mononuclear cells induced with the mitogens such as phytohemagglutinin (PHA) and pokeweed mitogen <i>in vitro</i> were significantly decreased on 3rd day after grouping compared with those on 3rd day before grouping in Group B. The intradermal response to PHA <i>in vivo</i> and the Phagocytotic function of monocytes were significantly reduced in all piglets until 14 days after grouping. In control piglets, a slight increase of plasma cortisol was also found, and the suppression of intradermal response to PHA was observed for 13 days.<br>These data suggested that grouping puts piglets under stress and suppresses the functions of lymphocytes and monocytes at least for 13 days after grouping.

Journal

  • Nihon Yoton Gakkaishi

    Nihon Yoton Gakkaishi 35(1), 1-8, 1998-03-10

    The Japanese Society of Swine Science

References:  26

Codes

  • NII Article ID (NAID)
    10008284881
  • NII NACSIS-CAT ID (NCID)
    AN10202971
  • Text Lang
    JPN
  • Article Type
    ART
  • ISSN
    0913882X
  • NDL Article ID
    4425470
  • NDL Source Classification
    ZR22(科学技術--農林水産--畜産)
  • NDL Call No.
    Z18-1082
  • Data Source
    CJP  NDL  J-STAGE  JASI 
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