Receptor-Mediated Facilitation of Cell Volume Regulation by Swelling-Induced ATP Release in Human Epithelial Cells

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Author(s)

Abstract

Osmotic swelling induces the release of intracellular ATP in a number of cell types. In the immediate vicinity of the cell surface, released ATP has been shown to reach a concentration high enough to stimulate P2-purinergic receptors in a human epithelial cell line, Intestine 407. The role of released ATP in the regulatory volume decrease (RVD) after cell swelling was thus studied in Intestine 407 cells. The RVD was suppressed by an ATP hydrolyzing enzyme, apyrase, or by a purinergic receptor antagonist, suramin. Extracellular application of ATP accelerated the RVD rate in a concentration-dependent manner. An increase in the cytosolic free-Ca<sup>2+</sup> concentration was induced by a hypotonic challenge, and the swelling-induced Ca<sup>2+</sup> response was partially suppressed by apyrase or suramin. A rise in cytosolic Ca<sup>2+</sup> was also induced by extracellular application of ATP or UTP, but not ADP, 2-methylthio-ATP or α,β-methylene ATP. The ATP-induced Ca<sup>2+</sup> response was blocked by suramin. Therefore, it is concluded that RVD is facilitated by ATP, which is released upon cell swelling, by augmenting intracellular Ca<sup>2+</sup> rise via the stimulation of purinergic (P2Y<sub>2</sub>) receptors in the human epithelial cell.<br>

Journal

  • The Japanese Journal of Physiology

    The Japanese Journal of Physiology 50(2), 235-241, 2000-04

    THE PHYSIOLOGICAL SOCIETY OF JAPAN

References:  31

Cited by:  3

Codes

  • NII Article ID (NAID)
    10008291036
  • NII NACSIS-CAT ID (NCID)
    AA00691224
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    0021521X
  • NDL Article ID
    5413519
  • NDL Source Classification
    ZS8(科学技術--医学--解剖学・生理学・生化学)
  • NDL Call No.
    Z53-D40
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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