Effects of Corticosteroids on Synaptic Transmission in Rat Dorsolateral Septal Nucleus

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Author(s)

Abstract

The effects of corticosteroids on synaptic transmission in the rat dorsolateral septal nucleus (DLSN) were examined, in vitro, by using intracellular and voltage-clamp recording methods. Prednisolone (100 μM) increased the amplitude of excitatory postsynaptic potential (EPSP) and depressed both fast and slow inhibitory postsynaptic potentials (IPSP). Under voltage-clamp conditions, prednisolone (100 μM) increased the amplitude of excitatory postsynaptic current (EPSC) and depressed the fast and slow inhibitory postsynaptic currents (IPSCs). Corticosterone (100 μM) mimicked the effects of prednisolone on the postsynaptic currents (PSCs). To examine the direct effects of prednisolone on the EPSC and slow IPSC, the fast IPSC was blocked by bicuculline (20 μM). Under these experimental conditions, prednisolone (100 μM) did not alter the isolated EPSC but depressed slow IPSC by 22 ± 3% (n = 10). The fast IPSC was isolated by pretreatment with kynurenic acid and CGP55845A, where the EPSC and slow IPSC were blocked. Prednisolone (100 μM) depressed the isolated fast IPSC in DLSN neurons. Prednisolone (100 μM) did not change either the inward current produced by glutamate or the outward current produced by γ-aminobutyric acid (GABA). The results suggest that corticosteroids facilitate excitatory synaptic transmission in the DLSN by reducing the release of GABA from the presynaptic nerve terminals of interneurons.<br>

Journal

  • The Japanese Journal of Physiology

    The Japanese Journal of Physiology 50(2), 267-272, 2000-04

    THE PHYSIOLOGICAL SOCIETY OF JAPAN

References:  27

Codes

  • NII Article ID (NAID)
    10008291162
  • NII NACSIS-CAT ID (NCID)
    AA00691224
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    0021521X
  • NDL Article ID
    5413549
  • NDL Source Classification
    ZS8(科学技術--医学--解剖学・生理学・生化学)
  • NDL Call No.
    Z53-D40
  • Data Source
    CJP  NDL  J-STAGE 
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