A Semi-Quantitative PCR Method for the Detection of Low Levels of Apoptotic DNA Fragmentation in a Heart Failure Model

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Author(s)

Abstract

Cells that are apoptotic and comprise less than 2% of the total cellular population are difficult to detect by conventional methods (i.e., DNA ladder). We discuss a new methodological technique, PCR-amplified DNA ladder, to detect very low levels of DNA fragmentation (indicative of apoptosis) in a myocardial infarct heart failure model. Results and methodology are contrasted with the traditional DNA ladder technique.<br>

Journal

  • The Japanese Journal of Physiology

    The Japanese Journal of Physiology 50(2), 281-284, 2000-04

    THE PHYSIOLOGICAL SOCIETY OF JAPAN

Codes

  • NII Article ID (NAID)
    10008291225
  • NII NACSIS-CAT ID (NCID)
    AA00691224
  • Text Lang
    ENG
  • Article Type
    SHO
  • ISSN
    0021521X
  • NDL Article ID
    5413575
  • NDL Source Classification
    ZS8(科学技術--医学--解剖学・生理学・生化学)
  • NDL Call No.
    Z53-D40
  • Data Source
    CJP  NDL  J-STAGE 
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