Effects of VEGF on Ca〔2+〕-Transient in Cultured Lymphatic Endothelial Cells and Mechanical Activity of Isolated Lymph Vessels

• Arai Fumi

  The 1st Department of Physiology, Shinshu University School of Medicine

• Mizuno Risuke

• Ohhashi Toshio

書誌事項

タイトル別名

• Effects of VEGF on Ca2+-Translent in Cultured Lymphatic Endothelial Cells and Mechanical Activity of Isolated Lymph Vessels.
• Effects of VEGF on Ca 2 Transient in Cultured Lymphatic Endothelial Cells and Mechanical Activity of Isolated Lymph Vessels

抄録

We investigated the effects of vascular endothelial growth factor (VEGF₁₆₅) on [Ca²⁺]_i-transient in cultured lymphatic endothelial cells (LEC) and mechanical activity of isolated dog thoracic ducts. VEGF (0.1-10 ng/ml) caused a dose-dependent increase of the [Ca²⁺]_i in LEC. Pretreatment with 10⁻⁵ m genistein or 5×10⁻⁶ m herbimycin A produced a significant reduction of the VEGF-induced [Ca²⁺]_i-transient. In the presence of 10⁻⁶ m thapsigargin, VEGF caused no significant effect on the [Ca²⁺]_i-transient. Pretreatment with Ca²⁺-free solution containing 0.1 mm EGTA produced no significant effect on the peak increase of [Ca²]_i induced by 0.1 or 10 ng/ml VEGF, but significantly depressed the sustained part of [Ca²⁺]_i observed at the higher concentration of VEGF. The VEGF (0.1-10 ng/ml) caused a significant dilation of the isolated lymph vessels with intact endothelium, which were precontracted with U46,619. The 10 ng/ml VEGF-induced dilation was significantly reduced by 3×10⁻⁵ m N^ω-nitro-l-arginine methyl ester (l-NAME). The action of l-NAME was inhibited by the simultaneous application of 10⁻³ m l-arginine. Mechanical rubbing of the endothelium also caused significant inhibition of the VEGF-induced dilation. The findings suggest that VEGF₁₆₅ may activate the receptor-related tyrosine kinase and cause the release of Ca²⁺ from the inositol 1,4,5-triphosphate-sensitive intracellular Ca²⁺ stores in LEC. VEGF₁₆₅ also produces endothelium-dependent nitric oxide-mediated dilation of the precontracted isolated lymph vessels.<br>

収録刊行物

• The Japanese Journal of Physiology

  The Japanese Journal of Physiology 50 (3), 343-355, 2000

  一般社団法人　日本生理学会

キーワード

• VEGF<SUB>165</SUB>
• lymphatic endothelial cell
• [Ca<SUP>2+</SUP>]<SUB>i</SUB>-transient
• nitric oxide
• dilation

詳細情報

• CRID: 1390001205041147008
• NII論文ID: 10008291575
• NII論文ID: 30015529656
• NII書誌ID: AA00691224
• DOI: 10.2170/jjphysiol.50.343
• COI: 1:CAS:528:DC%2BD3cXntFGju7s%3D
• ISSN: 18811396
• ISSN: 0021521X
• NDL書誌ID: 5525792
• PubMed: 11016985
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5525792
• Web Site: https://search.jamas.or.jp/link/ui/2001093847
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可