ATP Suppresses the K+ Current Responses to FSH and Adenosine in the Follicular Cells of Xenopus Oocyte.

• Fujita Reiko

  Department of Chemistry, School of Liberal Arts and Sciences, Iwate Medical University

• Kimura Shingo

• Kawasaki Satoshi

• Takashima Koichiro

• Matsumoto Mitsuhiko

• Hirano Hiroko

• Sasaki Kazuhiko

抄録

The application of either follicle-stimulating hormone (FSH) or adenosine (Ade) induces a K⁺-current response in the follicular cells surrounding a Xenopus oocyte under a voltage clamp. These K⁺-current responses are reported to be produced by an increase in intracellular cAMP. A previous application of ATP to the same cells markedly depressed the K⁺-current responses to FSH and Ade. Furthermore, a 2 min application of phorbol 12,13-dibutyrate (PDBu), an activator of protein kinase C (PKC), significantly depressed the K⁺-current responses to FSH and Ade, but it had no significant effect on the Cl⁻-current response to ATP. An application of either ATP or PDBu also depressed the K⁺-current response induced by intracellularly applied cAMP. In contrast to the effect of PDBu, the application of 1-octanol, an inhibitor of gap junction channel, significantly depressed both the Ade- and ATP-induced responses, indicating that the acting site of 1-octanol is different from that of PKC. The results suggest that the depressing effect of ATP on the FSH- and Ade-induced K⁺-current responses might be mediated by PKC activation and that the site of PKC action might be downstream of the cAMP production involved in the K⁺ channel opening.<br>

収録刊行物

• The Japanese Journal of Physiology

  The Japanese Journal of Physiology 51 (4), 491-500, 2001

  一般社団法人　日本生理学会

キーワード

• ATP
• FSH
• adenosine
• follicular cells
• <i>Xenopus</i> oocyte

詳細情報

• CRID: 1390001205040930560
• NII論文ID: 130004435781
• NII論文ID: 10008294481
• NII書誌ID: AA00691224
• DOI: 10.2170/jjphysiol.51.491
• COI: 1:CAS:528:DC%2BD3MXntlahsbk%3D
• ISSN: 18811396
• ISSN: 0021521X
• NDL書誌ID: 5907219
• PubMed: 11564286
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5907219
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可