胃癌細胞におけるsialyl Lewis抗原発現の臨床的意義 : フローサイトメトリー法による検討  [in Japanese] THE CLINICAL ROLE OF EXPRESSING SIALYL LEWIS ANTIGEN IN GASTRIC CANCER : FLOW CYTOMETRIC ANALYSIS  [in Japanese]

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Abstract

胃癌細胞におけるsialyl Lewis抗原の発現性を,肝転移の有無との関連性および予後因子としての意義について検討した.進行胃癌25例(肝転移9例,非肝転移16例)を対象とし,胃癌細胞と正常細胞各々約1-3万個のsLe<SUP>a</SUP>およびsLe<SUP>x</SUP>の発現率・発現量をモノクローナル抗体CA19-9,KM-93を用いフローサイトメトリーにより測定した.1)胃癌細胞のsLe<SUP>a</SUP>,sLe<SUP>x</SUP>の発現率・発現量は正常細胞と比較し有意(p<0.05)に高値であった.2)肝転移群胃癌細胞のsLe<SUP>a</SUP>発現事・発現量は非肝転移群と比較し有意(p<0.01)に高値であり,胃癌細胞のsLe<SUP>a</SUP>発現率・発現量と肝転移の有無との間に有意(p<記号0.01)な関連を認めた.3)sLe<SUP>a</SUP>の発現率は予後に対し有意(p<0.05)な影響を及ぼし,多変量解析より病理組織学的予後因子(pT,pN)と比較して優位な成績であった.以上より,フローサイトメトリー法によるsLe<SUP>a</SUP>の発現率は,胃癌の悪性度指標としての有用性が示唆された.

The purpose of this study was to compare the expression of sialyl Lewis antigens on the cell surface between gastric cancer and non-cancer cells and to elucidate possible roles of the antigens in predicting the development of liver metastasis. In addition, the usefulness as a prognostic factor was investigated. Subjects were 25 patients with advanced gastric cancer (nine with and 16 without liver metastasis). We measured sLea and sLex values expressed on the surface of about 10000-30000 cancer cells and non-cancer cells respectively for comparison. Monoclonal antibodies CA19-9 and KM-93 were used to evaluate the frequency and quantity of the expression by flow cytometry. The frequency and quantity of sLea and sLex in cancer cells were statistically higher than those in non-cancer cells (p<0.05). The frequency and quantity of sLea in cancer cells in the liver metastasis group was statistically higher than those in cases without liver metastasis (p<0.01). The values of expression of sLea on the surface of gastric cancer cells by flow cytometry indicated that there was a correlation with the development of liver metastasis (p<0.01). The frequency of sLea expression reflected clinical prognosis (p<0.05), and comparing to histopathological prognostic factors, it appeared to be superior to lymph node metastasis or depth of tumor invasion. It is strongly suggested that these values may be useful in evaluating the grade of malignancy.

Journal

  • Nihon Rinsho Geka Gakkai Zasshi (Journal of Japan Surgical Association)

    Nihon Rinsho Geka Gakkai Zasshi (Journal of Japan Surgical Association) 62(2), 332-340, 2001-02-25

    Japan Surgical Association

References:  21

Codes

  • NII Article ID (NAID)
    10008387903
  • NII NACSIS-CAT ID (NCID)
    AA11189709
  • Text Lang
    JPN
  • Article Type
    ART
  • ISSN
    13452843
  • Data Source
    CJP  J-STAGE 
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