Establishment of tsA58 Transgenic Rats as a Source of Conditionally Immortalized Cell Lines with Differentiated Functions

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Author(s)

Abstract

To isolate a variety of rat cell lines with differentiated functions, we developed transgenic rat lines that ubiquitously express the temperature-sensitive large T-antigen gene of the simian virus 40 (SV40) tsA58 mutant under the control of the SV40 large T-antigen promoter. These rats might be advantageous for simultaneously establishing cell lines from different tissues of rats with the same genetic origin. The transgenic rat lines transmit a functional copy of the transgene and were bred with sib mating to generate the homozygous transgene. The established cell lines from this transgenic rat had temperature dependent growth and retained some of the differentiated functions of each particular tissue, and were useful as a ready source of novel conditionally immortalized cell lines. The possible use and perspectives of these transgenic cell lines are discussed.

Journal

  • Experimental Animals

    Experimental Animals 51(2), 113-118, 2002-04

    Japanese Association for Laboratory Animal Science

References:  21

Cited by:  1

Codes

  • NII Article ID (NAID)
    10008394155
  • NII NACSIS-CAT ID (NCID)
    AA11032321
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    13411357
  • NDL Article ID
    6136287
  • NDL Source Classification
    ZS7(科学技術--医学)
  • NDL Call No.
    Z54-H752
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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