Simple Method for Assaying Serum Oxytocin and Changes of Serum Oxytocin Level during Parturition in Cynomolgus Monkeys

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Author(s)

Abstract

A novel and simple assay system using a 96-well ELISA plate was established for measuring serum oxytocin in cynomolgus monkeys. This method omits the centrifuge for B/F separation because the second anti-rabbit IgG antibody-coated ELISA plate can easily separate the first anti-oxytocin rabbit antibody-bound radiolabeled oxytocin. Since this method has the advantage of omitting B/F separation, it becomes possible to measure a large number of samples with simple steps. In addition, accurate and reproducible results could be obtained by this method. The optimal reaction condition made it possible to measure more than 8 pg/ml of serum oxytocin. The changes of serum oxytocin level in relation to the first delivery was determined in a total of 11 female monkeys who were divided into two groups, infant-accepting mothers (4 monkeys) and infant-rejecting ones (7 monkeys). The serum oxytocin levels of pre-delivery (one to 4 days before delivery) and post-delivery (within 12 hr after delivery) in infant-accepting mothers were 33.6 ± 4.57 and 43.5 ± 16.4 pg/ml, respectively. Those in infant-rejecting mothers were 39.0 ± 9.6 and 31.4 ± 7.0 pg/ml. Two-way ANOVA (accepting/rejecting × pre/post) revealed a significant interaction of two factors (F (1, 9)= 5.39, p<0.05). This result implies the possibility of a different pattern of oxytocin secretion between infant-accepting and infant-rejecting mothers during parturation.

Journal

  • Experimental Animals

    Experimental Animals 51(2), 181-185, 2002-04

    Japanese Association for Laboratory Animal Science

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Codes

  • NII Article ID (NAID)
    10008394350
  • NII NACSIS-CAT ID (NCID)
    AA11032321
  • Text Lang
    ENG
  • Article Type
    NOT
  • ISSN
    13411357
  • NDL Article ID
    6136402
  • NDL Source Classification
    ZS7(科学技術--医学)
  • NDL Call No.
    Z54-H752
  • Data Source
    CJP  NDL  J-STAGE 
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