Endothelium-Dependent Vasorelaxation Induced by Black Currant Concentrate in Rat Thoracic Aorta

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Author(s)

Abstract

We investigated the effect of black currant (BC) concentrate on smooth muscle in rat thoracic aorta. BC concentrate dose-dependently relaxed the norepinephrine (0.1 μM)-precontracted aorta, and the response was abolished after endothelium removal. Both oxyhemoglobin (1 μM), a nitric oxide (NO) scavenger, and 1<i>H</i>-[1,2,4]oxadiazolo-[4,3-<i>a</i>]quinoxalin-1-one (ODQ, 0.5 μM), an inhibitor of guanylyl cyclase (GC), inhibited the relaxing effect of BC concentrate. <i>N</i><sup>G</sup>-nitro-<sc><font size = -2>L</font></sc>-arginine methyl ester (<sc><font size = -2>L</font></sc>-NAME, 10 μM), a nitric oxide synthase (NOS) inhibitor, inhibited the relaxation, and the subsequent addition of <sc><font size = -2>L</font></sc>-arginine (1 mM), a NOS substrate, reversed the inhibitory effects of <sc><font size = -2>L</font></sc>-NAME. Neither indomethacin (10 μM), an inhibitor of cyclooxygenase, nor atropine (1 μM), an antagonist of muscarinic receptors, modified the effect of BC concentrate. Diphenhydramine (3 μM) and chlorpheniramine (2 μM), selective antagonists of H<sub>1</sub>-receptors, inhibited the relaxation, but cimetidine (0.3 mM), a selective antagonist of H<sub>2</sub>-receptors, did not affect the relaxation. These results indicate that, in the rat aorta, BC concentrate enhances synthesis of NO, which subsequently induces the endothelium-dependent vasorelaxation via the H<sub>1</sub>-receptors on the endothelium.

Journal

  • The Japanese Journal of Pharmacology

    The Japanese Journal of Pharmacology 89(1), 29-35, 2002-05-01

    The Japanese Pharmacological Society

References:  34

Cited by:  4

Codes

  • NII Article ID (NAID)
    10008430926
  • NII NACSIS-CAT ID (NCID)
    AA00691188
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00215198
  • NDL Article ID
    6169312
  • NDL Source Classification
    ZS51(科学技術--薬学)
  • NDL Call No.
    Z53-D199
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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