Synchrotron Radiation Microangiography System for Observation of Blood Flow in Murine Tumor Vasculature

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Author(s)

    • UMETANI Keiji
    • Life & Environment Division, Japan Synchrotron Radiation Research Institute
    • OGURA Masakazu
    • Department of Therapeutic Radiology and Oncology, Kyoto University

Abstract

The development of new blood vessels (angiogenesis) is important in the pathogenesis of rapid growth of solid tumors. Microangiography with a spatial resolution down to 8 μm was carried out at SPring-8 for the depiction of angiogenic vessels in a mouse model of cancer using a high-resolution image detector and a third-generation synchrotron radiation source. The new image detector is a fluorescent-screen optical-lens coupling system using a high-sensitivity pickup-tube camera. X-rays are converted into a visible image in a phosphor screen layer, and a visible light image is detected by the pickup tube. After analog-to-digital conversion, images are stored in a digital frame memory system with a 1024 × 1024-pixel and 10-bit format. In synchrotron radiation radiography, a long source-to-object distance and a small source spot can produce high-resolution images. A double crystal monochromator selected a single synchrotron radiation energy for imaging. Murine tumors had been transplanted into mice dorsal skin. Using a 9.0-keV monochromatic X-ray beam, small tumor blood vessels with diameters of 15 − 20 μm in an immature vascular network produced by angiogenesis were visualized after an iodine contrast material was injected into the common carotid artery.

Journal

  • bioimages

    bioimages 9(3), 97-106, 2001-12-01

    Bioimaging Society

References:  25

Codes

  • NII Article ID (NAID)
    10008449603
  • NII NACSIS-CAT ID (NCID)
    AA11084187
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    09192719
  • Data Source
    CJP  J-STAGE 
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