Synchrotron Radiation Microangiography System for Observation of Blood Flow in Tumor Vasculature

• Umetani Keiji

  Life & Environment Division, Japan Synchrotron Radiation Research Institute, SPring-8

• Itasaka Satoshi

  Department of Therapeutic Radiology and Oncology, Kyoto University School of Medicine

• Ogura Masakazu

  Department of Therapeutic Radiology and Oncology, Kyoto University School of Medicine

• Kimura Hiroyuki

  Department of Radiology, Hyogo Prefectural Amagasaki Hospital

• Hiraoka Masahiro

  Department of Therapeutic Radiology and Oncology, Kyoto University School of Medicine

Abstract

The development of new blood vessels (angiogenesis) is important in the pathogenesis of rapid growth of solid tumors. Microangiography with a spatial resolution down to 8 μm was carried out at SPring-8 for the depiction of angiogenic vessels in a mouse model of cancer using a high-resolution image detector and a third-generation synchrotron radiation source. The new image detector is a fluorescent-screen optical-lens coupling system using a high-sensitivity pickup-tube camera. X-rays are converted into a visible image in a phosphor screen layer, and a visible light image is detected by the pickup tube. After analog-to-digital conversion, images are stored in a digital frame memory system with a 1024 × 1024-pixel and 10-bit format. In synchrotron radiation radiography, a long source-to-object distance and a small source spot can produce high-resolution images. A double crystal monochromator selected a single synchrotron radiation energy for imaging. Murine tumors had been transplanted into mice dorsal skin. Using a 9.0-keV monochromatic X-ray beam, small tumor blood vessels with diameters of 15 − 20 μm in an immature vascular network produced by angiogenesis were visualized after an iodine contrast material was injected into the common carotid artery.

Journal

• bioimages

  bioimages 9 (3+4), 97-106, 2001

  Bioimaging Society

Keywords

• microangiography
• angiogenesis
• malignant tumor
• synchrotron radiation
• monochromatic X-ray

Details

• CRID: 1390282679415089536
• NII Article ID: 10008449603
• NII Book ID: AA11084187
• DOI: 10.11169/bioimages.9.97
• ISSN: 09192719
• Text Lang: en
• Data Source:
  • JaLC
  • CiNii Articles
• Abstract License Flag: Disallowed