Differentiating the Biosynthesis of Pseudomonic Acids A and B.
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- MANTLE PETER G.
- Department of Biochemistry, Imperial College of Science, Technology and Medicine
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- LANGEN MATTHIJS DE
- Department of Biochemistry, Imperial College of Science, Technology and Medicine
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- TEO VEI KIM
- Department of Biochemistry, Imperial College of Science, Technology and Medicine
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Abstract
Pseudomonic acid A (1) has been the dominant commercial pseudomonate antibiotic produced by Pseudomonas fluorescens. In specific shaken flask conditions initial fermentation accumulation of 1 is followed by preferential accumulation of the 8-hydroxy derivative, pseudomonic acid B (2). Biosynthetic probing with a pulse of [1-14C] acetate or L-[methyl-14C] methionine at early, mid and late stages of the fermentation gave relative patterns of radioactivity in 1 and 2 that are inconsistent with an assumption that 2 arises by oxidation of 1, or that 1 is formed by reduction of 2. Since [methyl-14C] methionine only labels carbons in the 12-carbon part of the pseudomonate molecule that is thought to be an early biosynthetic moiety, the evidence from radiolabelling experiments implies that preferential early oxidation of this biosynthetic intermediate causes the pathway diversion to accumulate 2 instead of 1.
Journal
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- The Journal of Antibiotics
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The Journal of Antibiotics 54 (2), 166-174, 2001
JAPAN ANTIBIOTICS RESEARCH ASSOCIATION
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Details 詳細情報について
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- CRID
- 1390001204150283648
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- NII Article ID
- 10008469798
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- NII Book ID
- AA0069330X
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- COI
- 1:CAS:528:DC%2BD3MXhsFyntrk%3D
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- ISSN
- 18811469
- 00218820
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- PubMed
- 11302490
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed