L-2,5-Dihydrophenylalanine, an Inducer of Cathepsin-dependent Apoptosis in Human Promyelocytic Leukemia Cells (HL-60).
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- KISO TETSUO
- Graduate School of Science, Osaka City University
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- USUKI YOSHINOSUKE
- Graduate School of Science, Osaka City University
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- PING XU
- Graduate School of Science, Osaka City University
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- FUJITA KEN-ICHI
- Graduate School of Science, Osaka City University
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- TANIGUCHI MAKOTO
- Graduate School of Science, Osaka City University
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Abstract
L-2, 5-Dihydrophenylalanine (DHPA), a phenylalanine analogue, induced apoptosis in human promyelocytic leukemia cells (HL-60). This apoptosis was demonstrated by morphological changes of the cells, such as fragmentation of nuclei and chromatin condensation, and by some evidence found in biochemical analysis, such as DNA ladder and activation of caspase 3. The DHPA-induced apoptosis was prevented by a pan-caspase inhibitor, Z-VAD-fmk, and a cysteine protease inhibitor, E-64d, which inhibits calpains and camepsin B and L. A calpain inhibitor, Z-LL-H, did not affect this apoptosis. A cathepsin B specific inhibitor, CA074-Me, prevented only chromatin condensation. However, E-64d and a cathepsin L specific inhibitor, Z-FY(t-Bu)-dmk, protected the cells from both chromatin condensation and oligonucleosomal DNA fragmentation. As proceeding to the apoptotic process, the activities of both cathepsin B and L increased gradually. These results indicated that DHPA was an inducer of cathepsin-dependent apoptosis in HL-60 cells.
Journal
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- The Journal of Antibiotics
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The Journal of Antibiotics 54 (10), 810-817, 2001
JAPAN ANTIBIOTICS RESEARCH ASSOCIATION
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Details 詳細情報について
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- CRID
- 1390282679126392448
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- NII Article ID
- 130003503823
- 10008471086
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- NII Book ID
- AA0069330X
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- COI
- 1:CAS:528:DC%2BD3MXnvF2gs7Y%3D
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- ISSN
- 18811469
- 00218820
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- PubMed
- 11776436
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- Text Lang
- en
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- Data Source
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- JaLC
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed