加齢および Alzheimer 病によるリンパ球幼若化能の変化 : WST-1法による測定  [in Japanese] The New Colorimetric Assay (WST-1) for Cellular Growth with Normal Aging and Alzheimer's Disease  [in Japanese]

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Abstract

WST-1法は, 細胞内の乳酸脱水素酵素などの関与する脱水素反応の活性を比色定量することを利用して, 間接的に細胞増殖能の程度を知る方法である.<br>今回, このWST-1法を, 同種の比色定量法であるMTT法と比較したところ, 簡便性, 正確さ, 感度の点からWST-1法のほうが優れていることが明らかになった.<br>そこで, WST-1法の使用例として, 健常者40例 (40~76歳) と Alzheimer 病患者16例 (47~74歳) を対象に, リンパ球をPHA-P刺激下で培養し, 幼若化の程度を測定した.<br>その結果, 健常者群では, リンパ球幼若化能の程度を表す比色値 (Y) と年齢 (X) の間にY=-0.0085X+1.473 (<i>r</i>=-0.3108, <i>p</i><0.05) の相関関係が成立することがわかった.<br>また, Alzheimer 病患者では, 加齢とともに比色値が増大する傾向が認められた.

A new tetrazolium compound, WST-1, a sodium salt of 4-[3-(4-iodophenyl)-2-(4-nitrophenyl)-2H-5-tetrazolio]-1, 3-benzendeisulfonate, was introduced into clinical chemistry. The compound's dehydrogenase activities are useful in the colorimetric determination of the bioreducibility of cells.<br>It proved to have a sensitivity similar to MTT, and produces a highly colored formazan dye under reductive conditions. Compared with the MTT assay, we found the WST-1 assay to be more sensitive, more convenient, and more exact. We used the new assay to study aging-related changes in human lymphocyte blastformation. Four groups of healthy adults aged 40-49 years (N=40), 50-59 years (N=40), 60-69 years (N=40), 70-76 years, (N=40) were examined. Moreover, we studied lymphocytes from 16 patients (47-74 years) with probable Alzheimer's disease.<br>For healthy controls, a negative correlation (<i>r</i>=-0.3108, <i>p</i><0.05) between age and enzyme activity was noted. The regression equation was:<br>Y=-0.0085X+1.473<br>where X and Y designate the age of the individual and enzyme activity (absorbance), respectively.<br>On the other hand, for patients with Alzheimer's disease, age and enzyme activity did not correlate.<br>These results indicate that the WST-1 assay may be useful in the study of changes associated with aging, and also more so than the MTT assay.

Journal

  • Nippon Ronen Igakkai Zasshi. Japanese Journal of Geriatrics

    Nippon Ronen Igakkai Zasshi. Japanese Journal of Geriatrics 35(7), 535-542, 1998-07-25

    The Japan Geriatrics Society

References:  16

Codes

  • NII Article ID (NAID)
    10008482367
  • NII NACSIS-CAT ID (NCID)
    AN00199010
  • Text Lang
    JPN
  • Article Type
    ART
  • ISSN
    03009173
  • NDL Article ID
    4555640
  • NDL Source Classification
    ZS21(科学技術--医学--内科学)
  • NDL Call No.
    Z19-25
  • Data Source
    CJP  NDL  J-STAGE 
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