<I>In Vitro</I> Translational Activity of Polysomal Polyadenylated mRNA Isolated from Seizure-susceptible EL Mouse Brain

• Nakanishi Aki

  Department of Neuropsychiatry, Osaka City University Medical School

Bibliographic Information

Other Title

• ELマウス脳から分離したpolysomal poly (A)<SUP>+</SUP>mRNAの<I>in vitro</I>の系での翻訳活性について

Abstract

We attempted to test protein molecules being translated by polysomal poly (A)⁺ m RNAs isolated from seizure-susceptible EL mouse brain. A significant lower translational activity was observed in both mice that experienced seizure (EL [s]) and those that did not (EL [ns]), compared to progenitor dd Y mice at 100 days of age. Anal ysis of translational products using a SDS-PAGE showed some quantitative differences in 28-and 100-day-old EL mice.Among 100-day-old mice, these proteins containing molecular weights of 23.5, 32.5 and 33.0kDa were not observed in EL [s] mice by analysis of two dimensional gel electrophoresis. The deficiencies of three proteins ma ybe associated with performed seizure-susceptibility due to toss-up stimulation approximately 40 times once a week. The data with regard to addition of exogenous poly (A) suggests that altered initiation during the translational process in protein synthesis takes place in EL mice compared with dd Y mice.

Journal

• Journal of the Japan Epilepsy Society

  Journal of the Japan Epilepsy Society 16 (3), 165-174, 1998

  JAPAN EPILEPSY SOCIETY

Keywords

• epilepsy
• EL mouse
• protein synthesis
• translation
• seizure-susceptibility

Details

• CRID: 1390282679492814208
• NII Article ID: 10008556033
• NII Book ID: AN10043823
• DOI: 10.3805/jjes.16.165
• ISSN: 13475509
• ISSN: 09120890
• Web Site: https://www.jstage.jst.go.jp/article/jjes1983/16/3/16_165/_pdf
• Web Site: https://search.jamas.or.jp/link/ui/1999081480
• Text Lang: ja
• Data Source:
  • JaLC
  • Crossref
  • CiNii Articles
• Abstract License Flag: Disallowed