cDNA cloning and characterization of two gelatinases from Japanese flounder.
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- KINOSHITA MASATO
- Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
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- YABE TAIJIRO
- Division of Molecular Oncology, Graduate School of Medicine (C7), Osaka University
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- KUBOTA MITSUTOSHI
- Division of Molecular Oncology, Graduate School of Medicine (C7), Osaka University
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- TAKEUCHI KAZUHARU
- Faculty of Agriculture, Kochi University
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- KUBOTA SATOSHI
- Faculty of Agriculture, Kochi University
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- TOYOHARA HARUHIKO
- Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
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- SAKAGUCHI MORIHIKO
- Division of Applied Biosciences, Graduate School of Agriculture, Kyoto University
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抄録
Toughness is one of the most important elements that define the commercial value of the raw meat of fish. Degradation of the extracellular matrix is thought to be a cause of postmortem tenderization of fish meat. A previous study has suggested that this tenderization is caused mainly by metalloproteinases. The present study seeks to identify the proteinase (s) involved in tenderization; hence, cloned cDNA of two gelatinases from Japanese flounder, which showed high homology with mammalian matrix metalloproteinase (MMP)-2 and MMP-9, were designated as jfMMP-2 and jfMMP-9, respectively. Northern blot analysis revealed that jfMMP-2 mRNA was expressed almost ubiquitously in adult tissues including the brain, muscle, gill, heart, gut, kidney, spleen, testis, and ovary. In contrast, the expression of jfMMP-9 mRNA was observed in those tissues which were abundant in blood cells, such as kidney, spleen, heart, and gill. Both recombinant proteins (jfMMP-2 and jfMMP-9) produced with the COS-7 cell system exhibited gelatin-degrading activity that was sensitive to 1, 10-phenanthroline, a typical metalloproteinase inhibitor.
収録刊行物
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- Fisheries science
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Fisheries science 68 (3), 618-626, 2002
公益社団法人 日本水産学会
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詳細情報 詳細情報について
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- CRID
- 1390001204428937728
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- NII論文ID
- 10008558196
- 130003903663
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- NII書誌ID
- AA10993718
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- COI
- 1:CAS:528:DC%2BD38Xlt1Wit7g%3D
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- ISSN
- 14442906
- 09199268
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- NDL書誌ID
- 6194911
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
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- 使用不可