ラットの低密度および高密度赤血球の過酸化リン脂質含量  [in Japanese] Phospholipid Hydroperoxide Contents of Low-and High-density Red Blood Cells in the Rat  [in Japanese]

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Abstract

ラットの赤血球 (RBC) をPercoll液を用いた密度勾配遠心法で低密度赤血球 (LD-RBC) と高密度赤血球 (HD-RBC) に分画し, そのリン脂質ハイドロパーオキサイド含量と食餌油脂の影響を調べた。その結果, ピルピン酸キナーゼ活性はHD-RBCでLD-RBCより低い傾向があり, 糖化ヘモグロビン含量は明らかにHD-RBCでLD-RBCより高く, HD-RBCは老化赤血球を多く含む画分と判断された。市販固形飼料 (F-2) 食, 大豆油食, 魚油食ラットともにLD-RBCとHD-RBCの過酸化リン脂質量に大きな差はなかった。しかし, 魚油食ラットの赤血球の過酸化リン脂質含量は大豆油食ラットより有意に高い値を示した。すなわち, 今回の実験から, ラットの赤血球において過酸化リン脂質の分布量はその密度 (老化度) に大きくは依存せず, むしろ食餌油脂の影響が大きく, とくに魚油の摂取は有意に赤血球の過酸化脂質を増やすことが明らかにされた。

High-density (HD-RBC) and low-density (LD-RBC) red blood cells were separated with Percoll solution (d 1.108) from rats fed for 28 days on commercial F-2 pellets, a soybean oil diet or a fish oil diet, and their phospholipid hydroperoxide contents were measured to investigate the peroxidation of RBC membranes in vivo. Rat RBC contained both phosphatidylcholine hydroperoxide (PCOOH, 50-127nM/packed RBC) and phosphatidylethanolamine hydroperoxide (PEOOH, 47-108nM/packed RBC) as the major hydroperoxides, and the peroxide content of RBC in rats fed fish oil was significantly higher than that in rats fed soybean oil. No difference in the peroxide content was observed between LD-RBC and HD-RBC. These results suggest that peroxidative damage to phospholipids in the RBC membrane is not as significant as expected during the aging of RBC, but that it is profoundly influenced by the type of dietary oil ingested. A fish oil diet causes more peroxidative damage to RBC membrane phospholipids than a soybean oil diet in the rat.

Journal

  • Nippon Eiyo Shokuryo Gakkaishi

    Nippon Eiyo Shokuryo Gakkaishi 48(2), 121-126, 1995-04-10

    Japan Society of Nutrition and Food Science

References:  23

Codes

  • NII Article ID (NAID)
    10008561789
  • NII NACSIS-CAT ID (NCID)
    AN00311992
  • Text Lang
    JPN
  • Article Type
    ART
  • ISSN
    02873516
  • NDL Article ID
    3619538
  • NDL Source Classification
    SC281(血液・造血器系)
  • NDL Source Classification
    ZS8(科学技術--医学--解剖学・生理学・生化学)
  • NDL Call No.
    Z19-53
  • Data Source
    CJP  NDL  J-STAGE 
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