高リン食投与時の腎石灰化と腎機能との関係  [in Japanese] The Relationship between Nephrocalcinosis and Kidney Function in Rats Fed High Phosphorus Diet  [in Japanese]

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Author(s)

    • 佐藤 茂 SATO Shigeru
    • 日本医科大学中央電子顕微鏡研究施設 Central Institute for Electron Microscopic Researches Nippon Medical School
    • 五島 孜郎 GOTO Shiro
    • 東京農業大学農学部栄養学科 Department of Nutrition, Faculty of Agriculture, Tokyo University of Agriculture

Abstract

Wistar系雄ラットにPレベル0.5% (正常食) と1.5% (高P食) を投与し, 尿中および腎臓中Ca, MgおよびP濃度と腎機能の指標として血清および尿中生化学的なパラメータを測定し, さらに組織学的検討を加え高P食投与時の腎石灰化と腎機能の関係について検討を試みた。<BR>1) 高P食投与により腎臓中Ca, Mg, P量および尿中P排泄量は増加した。腎機能の指標については, 血清中尿素窒素量, 尿中クレアチニン, アルブミン排泄量およびクレアチニンクリアランスが増加し, 尿素クリアランスは減少した。以上の結果から糸球体の機能低下を起こしていることが示唆された。<BR>2) 組織学的検討では, 高P食投与により一部の遠位尿細管管腔および大部分の集合管管腔が拡張し, その拡張した管腔の大部分に多量のCaの沈着が観察された。糸球体および近位尿細管ではCaの沈着および形態的変化は観察されなかった。

A study was undertaken to determine the relationship between nephrocalcinosis and kidney function in rats fed a high-phosphorus diet. Young male Wistar rats with an average initial body weight of 90g were used. The supplemental level of phosphorus in a basal diet was adjusted to 0.5% or 1.5%. The rats were then fed a diet containing either level of phosphorus for 21 days. Nephrocalcinosis was determined by analysis of kidney calcium content and histological examination of kidney tissue. As indicators of kidney function, urinary volume, serum and urine creatinine, albumin and urea nitrogen and creatinine and urea clearance were examined. The renal calcium content and urinary phosphorus excretion of rats fed the 1.5% phosphorus diet were higher than those of rats fed the 0.5% phosphorus diet. Urinary creatinine, and albumin excretion, serum urea nitrogen and creatinine clearance of rats fed the 1.5% phosphorus diet were higher than those of rats fed the 0.5% phosphorus diet, but urea clearance was decreased. In kidney tissue of the rats fed the 1.5% phosphorus diet, dilatation of part of the distal tubule and almost all of the collecting tubule was observed by light microscopy, and calcium deposition was revealed by von Kossa stainning.

Journal

  • Nippon Eiyo Shokuryo Gakkaishi

    Nippon Eiyo Shokuryo Gakkaishi 48(3), 217-223, 1995-06-10

    Japan Society of Nutrition and Food Science

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Codes

  • NII Article ID (NAID)
    10008562596
  • NII NACSIS-CAT ID (NCID)
    AN00311992
  • Text Lang
    JPN
  • Article Type
    Journal Article
  • ISSN
    02873516
  • NDL Article ID
    3619182
  • NDL Source Classification
    SC226;SC611
  • NDL Source Classification
    ZS8(科学技術--医学--解剖学・生理学・生化学)
  • NDL Call No.
    Z19-53
  • Data Source
    CJP  CJPref  NDL  J-STAGE 
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