Reduced von Willebrand Factor Fragment in Plasma
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- NONAMI Kyoko
- Department of Pediatrics, Nara Prefectural Gojo Hospital Department of Pediatrics, Nara Medical College
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- TAKAHASHI Yukihiro
- Division of Neonatology, Nara Medical College
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- NISHIKUBO Toshiya
- Department of Pediatrics, Nara Medical College
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- MORII Naoyuki
- Department of Pediatrics, Nara Medical College
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- YOSHIOKA Akira
- Department of Pediatrics, Nara Medical College
Bibliographic Information
- Other Title
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- 血漿 von Willebrand 因子の還元フラグメントの検討
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Abstract
Zimmerman et al reported that normal plasma vWF contains proteolytic fragments of 225, 189, 176, and 140 kDa with anti-vWF monoclonal antibodies in the presence of protease inhibitors and partial cleavage of the plasma vWF subunit is a normal event. Now we report here the analysis of normal plasma vWF with home-made polyclonal anti-reduced vWF antibody by using a small amount of normal plasma itself. With this reference, the vWF fragments with mobility corresponding to 240, 216 (220, 210), 194, 164, and 144 kDa were detected. Also by means of employing anti-reduced SP III and SP II antibody, we suggested that the 164 kDa fragment is located close to the carboxy terminal and the 194 and 144 kDa fragments are located close to the amino terminal. Furthermore, the vWF fragments showed no difference in mobility with or without protease inhibitor. In vivo proteolytic degradation of vWF occurring normally was also shown by our technique.
Journal
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- Japanese Journal of Thrombosis and Hemostasis
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Japanese Journal of Thrombosis and Hemostasis 6 (4), 279-286, 1995
The Japanese Society on Thrombosis and Hemostasis
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Details 詳細情報について
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- CRID
- 1390282679428322688
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- NII Article ID
- 130004262455
- 10008597543
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- NII Book ID
- AN10353762
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- ISSN
- 18808808
- 09157441
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- Data Source
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- JaLC
- Crossref
- CiNii Articles
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- Abstract License Flag
- Disallowed