Application of Mucin Histochemistry for Pathological Diagnosis : Expression of Gastric Phenotypes in Metaplastic and Neoplastic Lesions and its Relation to the Organoid Differentiation

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Author(s)

    • OTA Hiroyoshi
    • Department of Laboratory Medicine, Shinshu University School of Medicine
    • AKAMATSU Taiji
    • The second Department of Internal Medicine, Shinshu University School of Meidcine
    • FUJIMORI Yoshifumi
    • The second Department of Internal Medicine, Shinshu University School of Meidcine
    • MATSUZAWA Kenji
    • The second Department of Internal Medicine, Shinshu University School of Meidcine
    • ISHII Keiko
    • Department of Laboratory Medicine, Shinshu University School of Medicine
    • HONDA Takayuki
    • Department of Laboratory Medicine, Shinshu University School of Medicine
    • NAKAYAMA Jun
    • Department of Laboratory Medicine, Shinshu University School of Medicine
    • FURIHATA Kenichi
    • Department of Laboratory Medicine, Shinshu University School of Medicine
    • ONO Kenzo
    • Division of Pathology, Tosei General Hospital

Abstract

Using an array of histochemical and immunohistochemical techniques, we have characterized the various types of mucins in the normal, metaplastic and neoplastic stomach, pancreas and biliary ducts, in the normal and neoplastic esophagus, colon and lung, and in tumors of the ovary. Gastric phenotypes were variously expressed in metaplastic and neoplastic cells in these organs. Their expression was usually associated with an organoid differentiation simulating the gastric pyloric mucosa.<br>In gastric intramucosal carcinoma tissues, neoplastic cells often proliferated forming structures resembling non-neoplastic gastric mucosa. Thus, carcinoma cells in the more superficial layers expressed strong galactose oxidase cold thioneine Schiff reactivity (a surface mucous cell type property), whereas cells in the deeper layers were stained by paradoxical concanavalin A staining (a glandular mucous cell type property). Proliferative cells were perferentially located in the middle layers. This organized differentiation is lost in portions of the tumor that have invaded beyond the muscularis mucosae. Adenocarcinoma cells in both the bile duct and the pancreatic duct, in the bronchioloalveolar carcinoma of the lung and in mucinous cyst adenoma of the ovary showed gastric phenotypes and organoid differentiation. In papillary portions of these tumors, cells in protruded portions of the neoplastic papillae tended to express phenotypes of surface mucous cell, while in the indented portions they expressed phenotypes of glandular mucous cell. The goblet cell metaplasia in areas adjacent to pancreatic ductal carcinoma is identical to gastric pyloric metaplasia.<br>We conclude that the systematic use of mucin histochemistry, a relatively simple technique that can be implemented in most histopathology laboratories, may provide important information about structure and property of neoplastic tissues and could enhance our understanding of carcinogenesis in many organs.

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 28(1), 43-53, 1995-02-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

References:  79

Cited by:  3

Codes

  • NII Article ID (NAID)
    10008603455
  • NII NACSIS-CAT ID (NCID)
    AA00508022
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00445991
  • DOI
    10.1267/ahc.28.43
  • Data Source
    CJP  CJPref  J-STAGE 
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