Visualization of Acetylcholine in the Mouse Brain by a Combination of Immunohistochemistry with Ionic Fixation
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- TANAKA Jun-ichi
- Department of Anatomy, Kansai Medical University
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- MISHIRO Kayo
- Department of Anatomy, Kansai Medical University
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- WATANABE Jun
- Department of Anatomy, Kansai Medical University
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- KANAMURA Shinsuke
- Department of Anatomy, Kansai Medical University
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著者
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- TANAKA Jun-ichi
- Department of Anatomy, Kansai Medical University
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- MISHIRO Kayo
- Department of Anatomy, Kansai Medical University
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- WATANABE Jun
- Department of Anatomy, Kansai Medical University
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- KANAMURA Shinsuke
- Department of Anatomy, Kansai Medical University
抄録
Although acetylcholine (Ach) is an important neurotransmitter, there is no adequate histochemical method to detect Ach in tissue sections. We therefore developed a method to visualize the localization of Ach in tissue sections by a combination of immunohistochemistry with ionic fixation. Brains of mice, perfused with 4% formaldehyde and 5% phosphomolybdic acid (PMA) in 0.1 M phosphate buffer (pH 7.4) under sodium pentobarbital anesthesia, were removed and immersed quickly in boiling PMA solution for 5 min. Frozen sections were cut and soaked in a buffer containing 5% PMA at 4°C for 30 min, and immersed in a buffer containing bovine serum albumin at 4°C for 1 hr. Then the sections were incubated with anti-Ach antibody at 4°C for 12 hr. Subsequently, the immunoreaction due to Ach was visualized by the peroxidase-antiperoxidase method. Nerve cells in the brain sections from the interpeduncular nucleus of the cerebrum and bipolar cells in the multiform layer of the cerebral cortex were immmunohistochemically stained with the anti-Ach antibody.
収録刊行物
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- Acta histochemica et cytochemica
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Acta histochemica et cytochemica 28(3), 231-237, 1995-06-01
JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY
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