Visualization of Acetylcholine in the Mouse Brain by a Combination of Immunohistochemistry with Ionic Fixation

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Abstract

Although acetylcholine (Ach) is an important neurotransmitter, there is no adequate histochemical method to detect Ach in tissue sections. We therefore developed a method to visualize the localization of Ach in tissue sections by a combination of immunohistochemistry with ionic fixation. Brains of mice, perfused with 4% formaldehyde and 5% phosphomolybdic acid (PMA) in 0.1 M phosphate buffer (pH 7.4) under sodium pentobarbital anesthesia, were removed and immersed quickly in boiling PMA solution for 5 min. Frozen sections were cut and soaked in a buffer containing 5% PMA at 4°C for 30 min, and immersed in a buffer containing bovine serum albumin at 4°C for 1 hr. Then the sections were incubated with anti-Ach antibody at 4°C for 12 hr. Subsequently, the immunoreaction due to Ach was visualized by the peroxidase-antiperoxidase method. Nerve cells in the brain sections from the interpeduncular nucleus of the cerebrum and bipolar cells in the multiform layer of the cerebral cortex were immmunohistochemically stained with the anti-Ach antibody.

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 28(3), 231-237, 1995-06-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

References:  18

Codes

  • NII Article ID (NAID)
    10008603779
  • NII NACSIS-CAT ID (NCID)
    AA00508022
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    00445991
  • DOI
    10.1267/ahc.28.231
  • Data Source
    CJP  J-STAGE 
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