Detection of a Helicobacter pylori Gene Marker in Gastric Biopsy Samples by Non-radioactive in situ Hybridization

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Abstract

The presence of <I>H. pylori</I> was examined by non-radioactive <I>in situ</I> hybridization using a digoxigenin-labelled DNA probe and a synthetic <I>H. pylori</I> urease gene-specific thymine-thymine dimerized oligonucleotide probe. <I>H. pylori</I> was detected in 20/33 gastric biopsy specimens, and was localized in gastric epithelia and neutrophils. No nonspecific DNA signal was detected in colonic mucosa, esophageal mucosa, skin and liver specimens. The DNA probe did not crosshybridize with DNA from other bacteria. As this method is specific and not time-consuming, it may be useful for revealing the location of <I>H. pylori</I>.

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 28(3), 263-267, 1995-06-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

References:  29

Codes

  • NII Article ID (NAID)
    10008603874
  • NII NACSIS-CAT ID (NCID)
    AA00508022
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    00445991
  • Data Source
    CJP  J-STAGE 
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