Temporal Analysis of Increases in c-Fos Expression in Rat Spinal Trigeminal Nucleus Complex by Selective Stimulation of C-afferent Fibers

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Author(s)

    • TOMIOKA Toshiya
    • Second Department of Anesthesiology, Toho University School of Medicine

Abstract

This study evaluates temporal increases in the expression of Fos-like immunoreactive labeled neurons (FLI-labeled neurons) in the spinal trigeminal nucleus complex (STNC) following selective stimulation of C-fibers. Selective stimulating C-fibers were obtained using low doses (10 mg/kg b. w.) of capsaicin injection. Under sodium pentobarbital anaesthesia, 1% capsaicin was administered into the rats left infraorbital region. At periods of 30 min, 1, 3, 6, 9 and 12 hr after capsaicin injection, rats were anesthetized and killed for immunohistochemical examination of Fos-like protein products. The results showed that FLI-labeled neurons in the STNC appeared within 30 min after induction of capsaicin injection into the rat trigeminal region, reached peak increases at 3 hr, and disappeared between 9 and 12 hr. This time course was similar to dental treatment results which probably stimulated both A-delta and C fibers. According to previous studies, both A-delta and C fibers are essential to conduct impulses of nociceptive stimulation. But, this theory does not apply to the time course of FLI-labeled neuron expression in the STNC.<BR>In conclusion, this study showed that the time course of FLI-labeled neuron expression in the STNC was unchanged in stimulated or non-stimulated A-delta fibers.

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 28(3), 269-275, 1995-06-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

References:  31

Cited by:  1

Codes

  • NII Article ID (NAID)
    10008603904
  • NII NACSIS-CAT ID (NCID)
    AA00508022
  • Text Lang
    ENG
  • Article Type
    Journal Article
  • ISSN
    00445991
  • Data Source
    CJP  CJPref  J-STAGE 
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