Immunohistochemical and Immunochemical Localizaiton of Heat Shock Protein 27KD in the Developing Rat Retina: The Differential

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Abstract

The localization of two stress proteins, small heat shock protein (HSP27) and αB crystallin, in the developing rat retina were studied immunohistochemically and immunochemically, using the antibodies for HSP27 and αB crystallin. At embryonic day 15, the immunoreactivity to αB crystallin was observed in some cells in the neural retina and in the pigment epithelium (PE). At postnatal day (P) 5, immunoreactivity to HSP27 was first observed in the inner limiting membrane (ILM), nerve fiber layer (NFL) and PE. Immunoreactivity to αB crystallin was present in almost the same portions of the retina. At P10, the differentiating outer segments of the photoreceptor cells (ROS) and PE were intensely immunoreactive to αB crystallin, as compared with other layers, while the ILM, NFL and some portions of PE were immunoreactive to HSP27. At time progressed, immunoreactivity to HSP27 in the IPL and αB crystallin in the ROS increased. Our enzyme immunoassay of the retina revealed that the concentration of HSP27 gradually increased over time. In contrast, the concentration of αB crystallin increased after birth and reached almost to adult level at P30. The concentration of HSP27 was much lower as compared to that of αB crystallin. The differences in locations and changes in the levels of concentration between HSP27 and αB crystallin suggested that these two stress proteins may have different functions in the developing retina.

Journal

  • ACTA HISTOCHEMICA ET CYTOCHEMICA

    ACTA HISTOCHEMICA ET CYTOCHEMICA 28(4), 299-304, 1995-08-01

    JAPAN SOCIETY OF HISTOCHEMISTRY AND CYTOCHEMISTRY

References:  23

Codes

  • NII Article ID (NAID)
    10008604012
  • NII NACSIS-CAT ID (NCID)
    AA00508022
  • Text Lang
    ENG
  • Article Type
    ART
  • ISSN
    00445991
  • Data Source
    CJP  J-STAGE 
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