.ALPHA.-actinin-binding Protein (.ALPHA.-actinin) in Rat Cerebellar Cortex:Light and Electron Microscopic Immunolocalization.

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  • Gragera Raquel R.
    Department of Cellular Biology, Faculty of Biological Sciences, Cuidad Universitaria s/n., Complutense University of Madrid
  • Muñiz Enriqueta
    Department of Cellular Biology, Faculty of Biological Sciences, Cuidad Universitaria s/n., Complutense University of Madrid
  • Esteban Gema De
    Department of Cellular Biology, Faculty of Biological Sciences, Cuidad Universitaria s/n., Complutense University of Madrid
  • Martínez-Rodríguez Ricardo
    Department of Pathological Anatomy, C.I.C. Carlos III, National Institute of Health

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Abstract

α-actinin is an actin-binding protein, closely associated with actin-containing stress fibers and, in particular, with their membrane-bound terminals. Using the immunohistochemical pre-embedding PAP method, we have demonstrated the presence of actin-binding protein in the cerebellar cortex of adult rats at both light and electron microscopic levels. α-actinin immunoreactivity has been demonstrated within Bergmann glial cells, Golgi and stellate cells, astrocytes and cerebellar capillaries. These results suggest the essential involvement of this actin-binding protein in the cerebellar cortex function.

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